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EC number: 936-120-3 | CAS number: -
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- For this endpoint information from structural similar compounds (CAS 132123-39-8) is available. The studies for these similar compounds were performed according to GLP and the methods applied are fully compliant with OECD TG 301. See chapter 13 report for a more detailed justification.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 25 April - 02 July, 2001
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure):activated sludge, micro organisms from a domestic waste water treatment plant
- OriginMunicipal sewage treatment plant
- Conditioning: The activated sludge used for this study was washed by centrifugation and the supernatant liquid phase was decanted. The solid material was re-suspended in tap water and centrifuged again. This procedure was repeated twice.
- Concentration of suspended solids per litre: 30 mg - Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Parameter followed for biodegradation estimation:
- test mat. analysis
- Details on study design:
- According to guideline
- Reference substance:
- aniline
- Test performance:
- The reference item (Aniline) was sufficiently degraded to 77 % after 14 days and to 74% after 28 days of incubation.The validity criterion that the degradation should be > 60 % after 14 d was fulfilled.
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
- Key result
- Parameter:
- % degradation (test mat. analysis)
- Value:
- 0
- Sampling time:
- 28 d
- Results with reference substance:
- 14d: 77 %, 28d: 74 %
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The degradation rate of the test material did not reach 60 % within the 10-day window and after 28 days of incubation. Thus the test item is not readily biodegradable.
- Executive summary:
The test item was investigated for its ready biodegradability in a Manometric Respirometry Test over a period of 28 days. The biodegradation was followed by the oxygen uptake of the microorganisms during exposure. The substance concentration during the course of this study was monitored by LC with UV detection (254 nm). As a reference item aniline was tested simultaneously under the same conditions as the test item and functioned as a procedure control. The GLP study was performed according to OECD TG 301C.
Result
Biodegradation of test item
After correction of the mean biochemical oxygen demand of the inoculum controls at the end of the 28 -day exposure period a degradation of 0 % as found. HPLC analysis confirmed that no degradation occurs.
Biodegradation of aniline
The reference item was sufficiently degraded to 77 % after 14 days and to 74 % after 28 days of incubation, thus confirming the suitability of the used activated sludge inoculum.
Conclusion
The degradation rate of the test material did not reach 60 % within the 10 -day window and after 28 days of incubation. Thus, the test item is not readily biodegradable.
Referenceopen allclose all
Description of key information
OECD 301F (Read Across): not readily biodegradable
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
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