Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: The study has been performed according to OECD and EC guidelines and according to GLP principles.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2007
Report date:
2007

Materials and methods

Test guideline
Qualifier:
according to guideline
Principles of method if other than guideline:
OECD guidelines for Testing of Chemicals, guideline No. 202:
"Daphnia sp., Acute Immobilisation Test", Adopted April 13,
2004.


European Economic Community (EEC), EEC directive 92/69, Part
C: Methods for the determination of ecotoxicity,
Publication No. L383, December 1992, C.2. "Acute Toxicity
for Daphnia".


ISO International Standard 6341: "Water quality -
Determination of the inhibition of the mobility of Daphnia
magna Straus - Acute toxicity test, Third edition, 1996-04-
01.


Guidance document on aquatic toxicity testing of difficult
substances and mixtures, OECD series on testing and
assessment number 23, December 14, 2000.
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
-
EC Number:
481-490-7
EC Name:
-
Molecular formula:
multi-constituent.
IUPAC Name:
5,11,17,23,29,35,41,47-octa-tert-butyl-49,50,51,52,53,54,55,56-octahydroxy-2λ⁶,8λ⁶,14λ⁶,20λ⁶,26λ⁶,32λ⁶,38λ⁶,44λ⁶-octathianonacyclo[43.3.1.1³,⁷.1⁹,¹³.1¹⁵,¹⁹.1²¹,²⁵.1²⁷,³¹.1³³,³⁷.1³⁹,⁴³]hexapentaconta-1(49),3,5,7(56),9,11,13(55),15,17,19(54),21(53),22,24,27,29,31(52),33,35,37(51),39(50),40,42,45,47-tetracosaene-2,2,8,8,14,14,20,20,26,26,32,32,38,38,44,44-hexadecone; 5,11,17,23-tetra-tert-butyl-25,26,27,28-tetrahydroxy-2λ⁶,8λ⁶,14λ⁶,20λ⁶-tetrathiapentacyclo[19.3.1.1³,⁷.1⁹,¹³.1¹⁵,¹⁹]octacosa-1(25),3,5,7(28),9,11,13(27),15(26),16,18,21,23-dodecaen-2,2,8,8,14,14,20,20-octone
Details on test material:
- Name of test material (as cited in study report): TC4ASO2
- Substance type: multi-constituent
- Physical state: white powder
- Analytical purity: 97.8%
- Composition of test material, percentage of components: 92.9% 4-tert-Butylsulfonylcalix(4)arene (CAS 204190-49-8)
4.9% 4-tert-Butylsulfonylcalix(8)arene
- Lot/batch No.: AYUI-1Y
- Expiration date of the lot/batch: 07 October 2007
- Stability under storage conditions: Stable
- Storage condition of test material: At room temperature in the dark

Test solutions

Details on test solutions:
Identity and concentration of auxiliary solvent for dispersal: Test concentrations were prepared by stirring a dispersion
of the test substance in test medium (100 mg/l) for 72 hours
and subsequently filtering this mixture through a 0.45 mm
filter to obtain the water soluble fraction (WSF).
Additional test solutions (between 1% and 56%) were prepared
by dilution of various volumes of this filtrate in test
medium. All final test solutions were all clear and
colourless.

Test organisms

Test organisms (species):
other aquatic crustacea: DM

Study design

Test type:
static

Test conditions

Hardness:
180 mg CaCO3/L

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 0.08 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
0.08 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
%Concentration loss over test: ... 56

Any other information on results incl. tables

Comments:
Since 95% of the daphnids exposed to 100% WSF were recorded
as immobile in a combined limit/range-finding test, a final
test was performed exposing D. magna to 10, 18, 32, 56 and
100% of the WSF prepared at 100 mg/l. In contrast to the
range-finding test, exposure of D. magna to the 100% WSF did
not affect the mobility of more than 5% of the daphnids
exposed. In all other test solutions containing the WSF no
immobility of daphnids was recorded.


The initial concentration measured in the WSF was 0.18 mg/l
(final test), which decreased to 0.08 mg/l at the end of the
48-hour test period. Hence, the final concentration of 0.08
mg/l can be considered as the actual exposure concentration
in the final test.


The test substance did not induce acute immobilisation of
Daphnia magna, when these were exposed to a WSF prepared at
100 mg/l and corresponding with an actual concentration of
0.08 mg/l (NOEC).

Applicant's summary and conclusion