Registration Dossier
Registration Dossier
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EC number: 429-270-1 | CAS number: 136210-30-5
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
The effect of aspartic acid, N,N'-[methylenebis(2-methyl-4,1-cyclohexanediyl)]bis-, 1,1',4,4'-tetraethyl ester towards the growth of activated sludge was investigated according to ISO 8192 -1986 (E) having an EC50(3 h) of 3110 mg/L (Bayer AG, 1998d).
Key value for chemical safety assessment
- EC50 for microorganisms:
- 3 110 mg/L
Additional information
An adverse effect towards microorganisms and thus the cleaning capacity of sewage treatment plants is not expected based on the presented results.
Study was performed with aspartic acid, N,N'-[methylenebis(2-methyl-4,1-cyclohexanediyl)]bis-, 1,1',4,4'-tetraethyl ester which is a structural analogue to aspartic acid, N,N'-(methylenedi-4,1-cyclohexanediyl)bis-, 1,1',4,4'-tetraethyl ester. Both substances are diethyl esters of aspartic acid linked to a dicyclohexylmethyldiamine moiety. The difference between these two substances is merely the presence of two methyl groups connected to the cyclohexane rings. This structural analogy was confirmed by the Member State responsible for the notification of both substances under the NONS regulation. The Member State decided that test results obtained for one substance can be transferred to the other substance and that testing of both substances is usually not required. This decision is in accordance with the grouping of substances and read-across approach in Annex XI, 1.5 of the REACH Regulation.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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