Registration Dossier
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EC number: 429-270-1 | CAS number: 136210-30-5
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
Aspartic acid, N,N'-[methylenebis(2-methyl-4,1-cyclohexanediyl)]bis-, 1,1',4,4'-tetraethyl ester was tested with respect to the acute toxicity towards invertebrates (Daphnia magna) according to a national standard method: "Determination of the immobilization at daphnia", UBA, May 1984 yielding an EC0(48 h) ≥ 5 mg/L as no adverse effects were observed for testing concentrations up to the water solubility limit of the test item (Bayer, 1998c).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 5 mg/L
Additional information
According to the up-to-date criteria, the acute Daphnia test (Bayer AG, 1998) has strong restrictions, such as no monitoring of test concentrations for hydrolytically unstable substance and the presence of undissolved particles in the test vessels. Therefore the reported LC50(48h) of 88 mg/L, which is much higher than the expected water solubility of 5 mg/L, is not used for further hazard assessment. However, the observation of no adverse effects to Daphnia at the concentration of 10 mg/L, which is exceeding water solubility and hence assuming a saturated solution over the 48 h test duration, can be interpreted as the acute toxicity of aspartic acid, N,N'-[methylenebis(2-methyl-4,1-cyclohexanediyl)]bis-, 1,1',4,4'-tetraethyl ester towards Daphnia being higher than its water solubility (LC0(48 h) ≥ 5 mg/L).
Study was performed with aspartic acid, N,N'-[methylenebis(2-methyl-4,1-cyclohexanediyl)]bis-, 1,1',4,4'-tetraethyl ester which is a structural analogue to aspartic acid, N,N'-(methylenedi-4,1-cyclohexanediyl)bis-, 1,1',4,4'-tetraethyl ester. Both substances are diethyl esters of aspartic acid linked to a dicyclohexylmethyldiamine moiety. The difference between these two substances is merely the presence of two methyl groups connected to the cyclohexane rings. This structural analogy was confirmed by the Member State responsible for the notification of both substances under the NONS regulation. The Member State decided that test results obtained for one substance can be transferred to the other substance and that testing of both substances is usually not required. This decision is in accordance with the grouping of substances and read-across approach in Annex XI, 1.5 of the REACH Regulation.
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