Registration Dossier
Registration Dossier
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EC number: 278-860-3 | CAS number: 78182-00-0
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- November - March 1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 998
- Report date:
- 1999
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Qualifier:
- according to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Qualifier:
- according to guideline
- Guideline:
- other: EEC Directive 88/302/EEC (Activated Sludge Respiration Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- (2-hydroxyethyl)(3-hydroxypropyl)dimethylammonium chloride
- EC Number:
- 278-860-3
- EC Name:
- (2-hydroxyethyl)(3-hydroxypropyl)dimethylammonium chloride
- Cas Number:
- 78182-00-0
- Molecular formula:
- C7H18NO2.Cl
- IUPAC Name:
- 3-hydroxy-N-(2-hydroxyethyl)-N,N-dimethylpropan-1-aminium chloride
- Details on test material:
- - Name of test material (as cited in study report): DMAC Trocken Fluessig
- Chemical name: 1-Propanaminium, 3-hydroxy-N-(2-hydroxyethyl)-N, N-dimethyl-, chloride
- Molecular formular: C7 H18 NO2 Cl
- Batch no.: P 55
- Product no.: 097852
- Purity: no data (see Analytical Report ZAX No.: 98L00661)
- Molecular weight (g/mol): 183.7
- Aggregated state: solid
- Colour: light brown
- Water-solubility: miscible
- Instability against: heat > 100 °C
- Homogeneity: yes
- Remarks for storage: store at room temperature
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Inoculum: activated sludge from laboratory wastewater plants treating municipal and synthetic sewage
- Preparation of inoculum: inoculum was washed, brought to a concentration of 5 g/L dry substance and aerated for 24h. 50 ml were added to test
volume of 250 ml to obtain a concentration of 1 g/L dry substance in the test
- The concentration of dry substance was 1 g/l
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 30 min
Test conditions
- Test temperature:
- 20 +/- 2 °C
- pH:
- TEST SUBSTANCE
- pH-value before adding inoculum (after correction): 7.4
- pH-value after incubation time of 30 min: 7.5
REFERENCE SUBSTANCE
- pH-value before adding inoculum (after correction): 7.1 - 7.2 - Dissolved oxygen:
- TEST SUBSTANCE
- concentration at start (mg O2/L): 7.4
- concentration at end (mg O2/L): 5.3
REFERENCE SUBSTANCE:
- oxygen concentration at start (mg O2/L): 7.5 - 8.7
- oxygen concentration at end (mg O2/L): 5.5 - 8.4 - Nominal and measured concentrations:
- - test concentration (mg/L): 1000 (nominal)
- Details on test conditions:
- - test vessel: Erlenmeyer-vessel (nominal volume 250 ml)
- test volume: 250 ml
- synthetic medium: 8 ml/vessel 100-fold concentrated OECD Medium
- oxygen concentration during aeration: > 2.5 mg/L
- oxygen concentration immediately before measurement: > 6.5 mg/L
- duration of the measurement of oxygen consumption: 6 - 10 min
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- EC-VALUES:
duration: 30 min EC20: ca. 2.2 mg/L nominal test mat. respiration rate
duration: 30 min EC50: ca. 6.8 mg/L nominal test mat. respiration rate
duration: 30 min EC80: ca. 62 mg/L nominal test mat. respiration rate
highest concentration tested: 100 mg/L (nominal)
Applicant's summary and conclusion
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