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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

With high probability acutely not harmful to fish

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
224 mg/L

Additional information

Reliable results from three studies on the acute toxicity of 2 -dibutylaminoethanol (CAS 102 -81 -8) towards fish are available. A 96-h acute toxicity study was performed under static conditions according to German Industrial Standard DIN 38412, part 15, using Leuciscus idus as test species (BASF AG, 1987, report no. 10F0432/865014). The 96 -h LC50 was determined to be 31.6 mg/L (nominal; geometric mean; pH not adjusted). The toxicity was significantly reduced in pH-adjusted test solutions which resulted in an LC50 of 224 mg/L (geometric mean of LC0 = 100 mg/L and LC100 = 500 mg/L). The test concentrations were not analytically verified. However, DBEA proved to be stable in other studies (e.g. see IUCLID Chapters 6.1.3 or 6.1.5); in addition, the stubstance is assumed to be stable due to the substances' water solubility is 4000 mg/L (BASF SE 2012, report no. 11L00536), its moderate vapor pressure (VP = 0.05 hPa @20°C, BASF SE 2012, report no. 11L00536) and its low Henry's Law Constant (HLC = 0.217 Pa m³/mol, calculated with the VP/WS method, BASF SE 2017).

 

The results were supported by a GLP-study according to OECD 203, performed with Oryzias latipes as test species. The test conditions were semi-static. The guideline OECD 203 advises a pH adjustment if there is a strong change in the pH of the water after addition of the test substance. The pH strongly increased in this test after addition of the test substance (pH of fresh test solutions 10-100 mg/L: pH 8.5-9.8). The pH of the fresh test solutions was outside the recommended pH-range according to OECD 203 (pH 6.0 to 8.5). Therefore it cannot be excluded that the test substance is less toxic after pH-adjustment. Without pH-adjustment, 2-(dibutylamino)ethanol is acutely harmful to fish according to this test. The 96 -h LC50 was determined to be 29.2 mg/L (analytically verified; not pH-adjusted; NITE, 2004).

In another short-term acute toxicity test according to OECD 203, the 96 -h LC50 was determined to be 32 mg/L (nominal; not pH-adjusted) with Oryzias latipes as test species. This test was performed as a ranging-finding test within a bioaccumulation study according to OECD 305 and GLP (NITE, 2001). The test concentrations were not analytically verified but are assumed to be stable based on the assumptions given above.

The acceptable pH range for fish is given in the OECD TG 203 as pH 6 to 8.5. Without pH-adjustment the pH values exceeded this range; therefore, the data from these tests (BASF AG, 1987, without pH-adjustment; NITE, 2001; NITE, 2004a/2011) with fish should not be considered in the assessment of the DBEA as otherwise pH-effects cannot be distinguished from the inherent toxicity of DBEA to fish.

It is reasonable to conclude that DBEA is with high probability acutely not harmful to fish after pH-adjustment (96-h LC50 = 224 mg/L; BASF AG, 1987).