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Diss Factsheets
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EC number: 200-735-9 | CAS number: 70-47-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Genetic toxicity in vitro
Description of key information
-negative: Ames test, S.typhimurium TA98, TA 100, TA 102, TA 1535 and TA 1537 (met. act.: with and without) (OECD 471; GLP); reduction in number of revertants found in the negative control (exp.I, met. act.: with and without) was regarded as not biologically relevant due to lack of concomitant clearing of the background lawn, reduction in number of revertants down to 0.5 found in exp. II in strain TA 1535 at a concentration of 2500 µg/plate and in strain TA 1537 at concentrations of 316 and 1000 µg/plate (met. act.: without) was regarded as not biologically relevant due to lack of a dose-response relationship.
- negative: bacterial reverse mutation assay in E. coli strain SR250 (uvrB5) (met. act.: without) (similar to OECD 471, non-GLP); cytotoxicity: not reported.
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Genetic toxicity in vivo
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
A reverse gene mutation assay in bacteria according to OECD Test Guideline 471 is available for the test substance L-Asparagine. Also a reverse gene mutation assay in bacteria similar to OECD Test Guideline in E.coli is available as supporting data and there are published data that substantiate the notion that L-Asparagine is not associated with any genotoxic effect. In the study of Dyring et al. CHO cells were incubated with 10.2 and 5.8 mM L-Asparagine in order to determine the degree of metabolism in these cells. The increased ammonium content measured after incubation with L-Asparagine confirmed its metabolism. In another study of Costa et al. the induction of ornithine decarboxylase was determined in CHO cells incubated with 10 mM L-Asparagine. Although an increase of ODC activity was associated with an induction of cell proliferation, the treatment of CHO cells in this study did not result in an increased cell growth. In summary, the presented data provided evidence that L-Asparagine exhibits no genotoxic properties.
Justification for classification or non-classification
Based on the available, reliable and adequate data the substance does not need to be classified according to Regulation (EC) No 1272/2008 (CLP) and the Globally Harmonized System for Classification and Labeling of Chemicals (GHS) with respect to mutagenicity.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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