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EC number: 237-706-5 | CAS number: 13933-32-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Similar to OECD guidelines, with certain deviations.
- Remarks:
- Lacks a strain capable of detecting cross-linking mutagens, only tested at up to 1 mg/plate (instead of the recommended 5 mg/plate) and only plated in duplicate rather than in triplicate.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Report date:
- 1980
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- Guideline recommends the use of a strain capable of detecting cross-linking mutagens, which was not done. Only tested at up to 1 mg/plate (instead of the recommended 5 mg/plate). Only plated in duplicate rather than in triplicate.
- Principles of method if other than guideline:
- Method carried out according to Ames et al. (1975). Mutation Research 31, 347-364.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Tetraammineplatinum dichloride
- EC Number:
- 237-706-5
- EC Name:
- Tetraammineplatinum dichloride
- Cas Number:
- 13933-32-9
- Molecular formula:
- Cl.1/2H12N4Pt
- IUPAC Name:
- Tetraammineplatinum dichloride
- Details on test material:
- - Name of test material (as cited in study report): only cited by molecular formula Pt(NH3)4Cl2
- Substance type: cream powder
- Physical state: solid
- Analytical purity: no data
- Impurities (identity and concentrations): no data
- Purity test date: no data
- Lot/batch No.: 2 batches used – 031099/A and “uncoded”
- Expiration date of the lot/batch: no data
- Stability under test conditions: no data
- Storage condition of test material: no data on storage of test substance; solutions prepared immediately before testing
- Other:
Constituent 1
Method
- Target gene:
- histidine
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535
- Additional strain / cell type characteristics:
- not applicable
- Species / strain / cell type:
- S. typhimurium TA 1537
- Additional strain / cell type characteristics:
- not applicable
- Species / strain / cell type:
- S. typhimurium TA 98
- Additional strain / cell type characteristics:
- not applicable
- Species / strain / cell type:
- S. typhimurium TA 100
- Additional strain / cell type characteristics:
- not applicable
- Species / strain / cell type:
- S. typhimurium TA 1538
- Additional strain / cell type characteristics:
- not applicable
- Metabolic activation:
- without
- Metabolic activation system:
- Rat liver microsomal fraction (S9) from male, CD1 rats induced with Aroclor 1254
- Test concentrations with justification for top dose:
- 1.6, 8.0, 40, 200 and 1000 µg/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: deionised water (uncoded batch); isotonic saline (batch 031099/A)
- Justification for choice of solvent/vehicle: no data
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: N-methyl-N-nitro-N- nitrosoguanidine
- Remarks:
- 10 µg/plate for TA1535 and TA100 without S9
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene
- Remarks:
- 20 µg/plate for TA1537, TA 98 and TA100, with and without S9
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- 50 µg/plate for TA1537 without S9
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- 20 µg/plate for TA1538 and TA98 without S9
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Exposure duration: 48 hr
OTHER: Plates prepared in duplicate.
Only tested up to 1 mg/ml although no problem with solubility was reported.
Tests with TA1537, TA98 and TA100 were conducted twice, both with and without S9 using the “uncoded” test compound in water. Three separate tests were performed with batch 031099/A dissolved in isotonic saline and TA1535, TA1537 and TA1538, only without S9. - Evaluation criteria:
- No data, but reported the method to be according to Ames et al. (Mutation Research 1975, 31, 347-364), who considered a less than 2-fold increase in revertants, compared to spontaneous revertants, to be a negative response.
- Statistics:
- No data
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- other: weak positive
- Cytotoxicity / choice of top concentrations:
- other: cytotoxic at 1000 μg/plate in one of the duplicate tests, both in the presence and absence of S9
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- other: weak positive
- Cytotoxicity / choice of top concentrations:
- other: cytotoxic at 1000 μg/plate in one of the duplicate tests, both in the presence and absence of S9
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
TEST-SPECIFIC CONFOUNDING FACTORS No data
RANGE-FINDING/SCREENING STUDIES: no data
COMPARISON WITH HISTORICAL CONTROL DATA: no data in the report [but revertants within the ranges given in the literature].
ADDITIONAL INFORMATION ON CYTOTOXICITY: in one of the duplicate tests there was a thinning of the background lawn reported for TA98 and TA100 together with a decrease in the number of revertants, both with and without S9. This was not evident with TA1537, or for TA 1535 and TA 1538 which were only tested without S9.
|
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
positive with metabolic activation
positive without metabolic activation
In a limited study, tetraammineplatinum dichloride showed a dose-related mutagenic activity in TA 1537, and a weak positive response (about a 2-fold increase in mutant frequency, compared to the spontaneous frequency) in TA98 and TA100, both in the presence and absence of metabolic activation. - Executive summary:
Tetraammineplatinum dichloride was tested for mutagenic activity by reversion to histidine independence in five strains of Salmonella typhimurium, TA1535, TA1537, TA 1538, TA98 and TA100, using pour-plate assays.
Duplicate assays were carried out with strains TA98, TA100 and TA1537, both with and without a rat liver microsomal fraction (S9), using an aqueous solution of the test item. Three separate tests were conducted with strains TA1535, TA1537 and TA1538 in the absence of S-9 mix only, using the test substance dissolved in isotonic saline solution. In all experiments, test doses ranged from 1.6 to 1000 µg/plate (lower than the recommended top concentration).
A dose-related increase in mutant frequency (13- to 36-fold above that for spontaneous mutants) was observed in TA 1537, which detects frameshift mutations. A weak positive response (about a 2-fold increase in mutant frequency, compared to the spontaneous frequency) was also observed in TA98 and TA100, which also detect frameshift mutations. These increases in mutant frequencies were seen both in the presence and absence of S9.No increase was seen with strains TA1535 and TA1538, either in the presence or absence of S9.
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