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EC number: 950-480-9
CAS number: -
Table 2 Lethal effect of Algea Fert Solid K+ toDaphnia
Nominal test item concentration
No. of immobilizedDaphnia
% of immobilizedDaphnia
0 (negative control)
The acute toxicity of the test item Algea Fert Solid K+ toDaphnia
magna was determined in a 48‑hour static test according tothe OECD
Guideline for Testing of Chemicals, No. 202 (2004) and to Council
Regulation EC 440/2008 (C.2).
For this purpose, juvenile daphnids (<24 hours old at initiation
of the test) were exposed to an aqueous test medium containing the test
item at the only nominal concentration of 100.0 mg/L. The exposed
organisms were checked for immobilization 24 and 48 hours after test
The pH of the control (test medium without test item) was 7.36 and
the pH of test item solution immediately after preparation was 7.51,
therefore, the pH of the test medium was not adjusted before the start
of the test.
The room temperature was in the range 21.2 –during the test
period, according to the OECD’s recommended range (20 ± 2 °C).
Light intensity during the 48 hours of test period was in the
range 385-410 Lux, within the range 200-1500 Lux.
At the end of test period, in the negative control no
immobilization was observed and no daphnid was found trapped on the test
water surface. These values comply with the specified validity criterion
that immobilization in the negative control group should not exceed 10%
at the end of the test.
No immobilization was observed in the test item medium after
24 and 48 hours, therefore the NOEC (highest concentration tested
without toxic effects after the exposure period) of Algea Fert Solid K+
to Daphnia magna was determined to be equal to the nominal tested
concentration of 100.0 mg/L.
Based on the observed effects, the LOEC and the 48-h IC50were
determined to be greater than the tested concentration, 100.0 mg/L.
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