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Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Short term toxicity to aquatic invertebrates:

By applying weight of evidence approach the EC50 value of test chemical was determined to be >100 mg/L after exposure of test chemical for 48 hrs. The EC50 value indicates that the substance is likely to be nonhazardous to aquatic invertebrates and cannot be classified as per the CLP classification criteria

Toxicity to aquatic algae:

By applying weight of evidence approach the EC50 value of test chemical was determined to be in range from 190 mg/L to 276.1 mg/l after exposure of test chemical for 72 hrs. The range EC50 value indcates that the substance is likely to be nonhazardous to aquatic algae and cannot be classified as per the CLP classification criteria.

Additional information

Short term toxicity to aquatic invertebrates:

Data available for the structurally and functionally read across chemicals has been reviewed to determine the short term toxicity to aquatic invertebrates. The studies are as mentioned below:

The first study reviewed from the secondary source and this study was performed to determine the effect of test chemical on Daphnia magna according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). The vehicle used in the study was DMSO and HCO-40 at ratio of DMSO: HCO-40 is 9:1 (100 mg/l), and laboratory water was used as negative control.

The test was performed in static system. The 5 nominal concentrations of test chemical were used and they are in range of 90-940 mg/l. The static procedure was used in this study and nominal concentrations of test chemical used were exposed to Daphnia magna for 24 hrs.

The effect concentration of test chemical at which 50 % of Daphnia magna were immobilized was determined to be 280 mg/l with 95% confidence level of 150-490 mg/L.

Another study was performed to determine the effect of test chemical on Daphnia magna according to OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test). The test chemical solution 100.0 mg/L was prepared by dissolving test chemical in reconstituted water. The solution was kept 5 min in ultrasonic bath. Limit test was performed in this study at concentration 100 mg/L. The positive control used was Potassium dichromate (K2Cr2O7). Static system was used in this study and test chemical was exposed to Daphnia magna for 48 hrs. The median effective concentration (EC50) of test chemical, in Daphnia magna was determined to be >100 mg/L. Based on this EC50 value test chemical is considered to be non toxic to Daphnia Magna and cannot be classified as per CLP regulation.

Aim of this next study was to assess the short term toxicity of test chemical to aquatic invertebrate daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. The test chemical solution of concentration 100 mg/L was prepared by dissolving test chemical in reconstituted water. Limit test was performed in this study at concentration 100 mg/L .Effects on immobilization were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, inhibition percentage (I%) was calculated using nonlinear regression by the software Prism 4.0. The inhibition percentage (I%) for the test substance in Daphnia magna was determined to be 8.0 % at 100 mg/L on the basis of mobility inhibition effects . Based on the percentage (I%) value, substance is considered to be non-hazardous to aquatic invertebrate and cannot be classified as per the CLP classification criteria.

By considering results of both the studies mentioned above and applying weight of evidence approach the EC50 value of test chemical was determined to be >100 mg/L after exposure of test chemical for 48 hrs. The EC50 value indicates that the substance is likely to be nonhazardous to aquatic invertebrates and cannot be classified as per the CLP classification criteria

Toxicity to aquatic algae:

Data available for the structurally and functionally read across chemicals has been reviewed to determine the toxicity to aquatic algae. The studies are as mentioned below:

In first study the freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the test chemical according to OECD Guideline 201 (Alga, Growth Inhibition Test). The stock solution 200 mg/L was prepared by dissolving test chemical in OECD growth medium. The stock solution was kept 5 min in ultrasonic bath. Test solutions of required concentrations were prepared by mixing the stock solution of the test chemical with OECD growth medium. The nominal concentration used in this study was 12.5, 25, 50, 100 and 200 mg/L. Static system was used in this study and test chemical was exposed to green algae for 72 hrs. The test vessel used was 50 ml glass vessel. Effects on the growth rate of the Desmodesmus subspicatus were studied. ErC50 calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (ErC50) for the test chemical on a freshwater green algae Desmodesmus subspicatus was determined to be 276.1 mg/L. On the basis ErC50 value after 72 hour exposure of test chemical to green algae test chemical is considered to be non- toxic and cannot be classified as per the CLP classification criteria.

Another study was reviewed from secondary was performed to determine the toxicity of test chemical, to algae according to the standard OECD Guideline 201 (Alga, Growth Inhibition Test). Test organism used was Selenastrum capricornutum ATCC 22662. 13 nominal concentrations of test chemical were used in this study in range of 1.0-1000 mg/l. The test was performed in static open system by following the OECD guideline. Selenastrum

capricornutum were exposed to the chemical for 72hrs.

Based on the biomass inhibition of algae by the test chemical, the EC50 value and NOEC value was determined to be 190 mg/L and 5.8 mg/L, respectively. Thus, based on the EC50 value it was concluded that the test chemical was nontoxic and can be considered to be not classified as per the CLP classification criteria.

By considering results of both the studies mentioned above and applying weight of evidence approach the EC50 value of test chemical was determined to be in range from 190 mg/L to 276.1 mg/l after exposure of test chemical for 72 hrs. The range EC50 value indcates that the substance is likely to be nonhazardous to aquatic algae and cannot be classified as per the CLP classification criteria.