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EC number: 214-946-9 | CAS number: 1222-05-5
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
The 96h-LC50 to Medaka larvae according to a study equivalent to OECDTG 203, with semi-static exposure was 0.95 mg/l based on nominal concentrations.
Key value for chemical safety assessment
Additional information
Two fish toxicity studies are available. The study with larvae from Medaka (Oryzias latipes) study by Yamauchi (2008) is used as the key study. The study by Carsson and Norrgren (2004) is summarised but has been disregarded.
Key study
Yamauchi (2008), freshwater, Medaka larvae, Aquatic Toxicology 90: 261–268: In the key study, the acute toxicity of fish larvae was examined in a study equivalent to OECD TG 203. In this study 24h-old Medaka larvae (Oryzias latipes) were exposed to the substance for 96 hours. Fifteen larvae were exposed to concentrations of the substance ranging from 0.60 -1.5 mg/l substance. The test solutions were refreshed on a daily basis. DMSO was used as a solvent carrier and a control with 0.1% DMSO was therefore included. Results: The 96h-LC50 was found to be 0.95 mg/l (95% CL: 0.91 - 1.01 mg/l) based on nominal concentrations.
Another fish study on Zebrafish embryos was disregarded because the biological significance of this parameter however is unclear and it is unsuitable for risk assessment (EU RAR, 2008). Due to insufficient information on the experimental details including actual concentration, the study has been disregarded. Source: EU Risk Assessment Report (2008)
Carlson and Norrgren (2004), freshwater, zebrafish, Arch. Environ, Contam Toxicol 46: 102-105: A short-term embryo toxicity study was carried out by Carlson and Norrgren. In this study 20 zebrafish (Brachydanio rerio) per exposure group were exposed to nominal concentrations ranging between 1 to 1000 µg/l under static conditions for 96 hours. Concentrations were not measured. Selected newly laid zebrafish eggs were individually exposed in 96-well styrene plates in 250 µl of test medium for 48 hours. Results: No effects were found on development of the embryos after two days. A slight but not significant effect was found on heart rate that was decreased by 5% in 1000 µg/l based on nominal values.
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