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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Description of key information

The chronic toxicity of 4 -Hydroxydiphenylamine to Daphnia magna was investigated in a static test according to Council Regulation (EC) No 440/2008, Method C.20 'Daphnia magna Reproduction Test'' (2008), which equals to the OECD Test guideline 211 (Daphnia magna Reproduction Test; 1998). After 21 days a NOEC of 0.028 mg/L was determined. 

Key value for chemical safety assessment

EC10, LC10 or NOEC for freshwater invertebrates:
0.028 mg/L

Additional information

4-Hydroxydiphenylamine (4 -HPDA) is the hydrolysis product from N-(1,4-dimethylpentyl)-N'-phenylbenzene-1,4-diamine (7PPD). A secondary hydrolysis product is p-hydroquinone.


The lowest effect value in long-term tests was found for p-hydroquinone (0.007 mg/L, expressed in 7PPD equivalents) which is lower than the value for 4-HDPA (0.028 mg/L).Although p-hydroquinone is a secondary hydrolysis product of 7PPD, it was not chosen as a source of key value as the formation of p-Hydroquinone from 7PPD by hydrolysis is rather slow.