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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from authoritative database and secondary source
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
GLP compliance:
not specified
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
- Concentration of sludge: 100 mg/l
Duration of test (contact time):
28 d
Initial conc.:
30 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
TOC removal
Parameter followed for biodegradation estimation:
other: BOD and UV-Vis
Details on study design:
Standard type
Key result
Parameter:
other: BOD
Value:
74
Sampling time:
28 d
Remarks on result:
other: Other details not known
Key result
Parameter:
% degradation (TOC removal)
Value:
44
Sampling time:
28 d
Remarks on result:
other: Other details not known
Key result
Parameter:
other: UV-Vis
Value:
62
Sampling time:
28 d
Remarks on result:
other: Other details not known
Details on results:
Test substance undergoes 74, 44 and 62% degradation by BOD, TOC removal and UV-Vis parameter in 28 days.
Validity criteria fulfilled:
not specified
Interpretation of results:
readily biodegradable
Conclusions:
The percentage degradation of test chemical was determined to be 74, 44 and 62% by BOD, TOC removal and UV-Vis parameter in 28 days. Thus, based on percentage degradation, test chemical is considered to be readily biodegradable in water.
Executive summary:

Biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test chemical. The study was performed according toOECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I) under aerobic conditions. Activated sludge was used as a test inoculum for the study. Concentration of inoculum i.e, sludge used was 100 mg/l and initial test substance conc. used in the study was 30 mg/l, respectively. The percentage degradation of test chemical was determined to be 74, 44 and 62% by BOD, TOC removal and UV-Vis parameter in 28 days. Thus, based on percentage degradation, test chemical is considered to be readily biodegradable in nature.

Description of key information

Biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test chemical (from authoritative database, 2018 and secondary source, 2014). The study was performed according toOECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I) under aerobic conditions. Activated sludge was used as a test inoculum for the study. Concentration of inoculum i.e, sludge used was 100 mg/l and initial test substance conc. used in the study was 30 mg/l, respectively. The percentage degradation of test chemical was determined to be 74, 44 and 62% by BOD, TOC removal and UV-Vis parameter in 28 days. Thus, based on percentage degradation, test chemical is considered to be readily biodegradable in nature.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

Various experimental studies of the test chemical were reviewed for the biodegradation end point which are summarized as below:

 

In an experimental study fromauthoritative database (2018) and secondary source (2014), biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test chemical. The study was performed according to OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I) under aerobic conditions. Activated sludge was used as a test inoculum for the study. Concentration of inoculum i.e, sludge used was 100 mg/l and initial test substance conc. used in the study was 30 mg/l, respectively. The percentage degradation of test chemical was determined to be 74, 44 and 62% by BOD, TOC removal and UV-Vis parameter in 28 days. Thus, based on percentage degradation, test chemical is considered to be readily biodegradable in nature.

 

Another biodegradation study was conducted for 30 days for evaluating the percentage biodegradability of test chemical (HSDB, 2017). The study was performed according to OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test) under aerobic conditions. Activated sludge was used as a test inoculum for the study. Initial test substance conc. used in the study was in the range 2-5 mg/l, respectively. The percentage degradation of test chemical was determined to be 74-84% by BOD parameter in 28 days. Thus, based on percentage degradation, test chemical is considered to be readily biodegradable in water.

Estimation Programs Interface Suite (2018) was run to predict the biodegradation potential of the test chemical in the presence of mixed populations of environmental microorganisms. The biodegradability of the substance was calculated using seven different models such as Linear Model, Non-Linear Model, Ultimate Biodegradation Timeframe, Primary Biodegradation Timeframe, MITI Linear Model, MITI Non-Linear Model and Anaerobic Model (called as Biowin 1-7, respectively) of the BIOWIN v4.10 software. The results indicate that test chemical is expected to be readily biodegradable.

 

On the basis of above results of test chemical, it can be concluded that the test chemical is considered to be readily biodegradable in water.