[No public or meaningful name is available]

Toxicity to terrestrial arthropods

Administrative data

Endpoint:

toxicity to terrestrial arthropods, other

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

2009

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Data source

Reference

Reference Type:

publication

Title:

Influence of a Juvenile Hormone Analog and Dietary Protein on Male Anastrepha suspensa (Diptera: Tephritidae) Sexual Success

Author:

R. Pereira, J.Sivinski, P.E.A. Teal

Year:

2010

Bibliographic source:

J. Econ. Entomol. 103(1): 40-46 (2010);

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

The goal of the study was to determine the effects of a juvenile hormone analog (methoprene) application, of protein in the adult diet, and their interactions on the sexual performance of males of the Caribbean fruit fly, Anastrepha suspensa (Loew). Specifically, male competitiveness in agonistic interactions with competing mates, attractiveness to females, and mating success were measured. Experiments were conducted in laboratory and field cages and on daily and lifetime
bases.

GLP compliance:

not specified

Remarks:

published data

Application method:

oral

Test material

Test material information

Constituent 1

Reference substance name:

yeast protein hydrolysate

IUPAC Name:

yeast protein hydrolysate

Test substrate

Details on preparation and application of test substrate:

hydrolyzed protein was added to the sugar diet in a proportion of three parts of sugar and one part of hydrolyzed yeast protein.

Test organisms

Test organisms (species):

other: Anastrepha suspensa

Animal group:

Diptera (fruit fly (small))

Study design

Study type:

other: Laboratory study and field study

Total exposure duration:

35 d

Test conditions

Test temperature:

Laboratury study: 25 +/-1 deg C
Field study: 24 - 30 deg C

Humidity:

Laboratury studyL 55+/-5%
Field study: 48 - 79%

Photoperiod and lighting:

Laboratury study: photoperiod of 13 L:11D(light from 07:00 to 20:00 h), with light intensity of 550+/- 50 lux

Field study: Light intensity dropped from 1,112 +/- 135 lux at 17:00 to 291 +/- 71 lux at 19:00 h. The maximum light intensity was registered on 12 October at 17:00 hours (1,345 lux). Sunset occurred at 19:11 h on 4 October (first day of experiment) and at 19:00 h on 13 October (last day of experiment).

Nominal and measured concentrations:

25% of the diet

Results and discussion

Details on results:

Both laboratory and field cage tests found a clear increase in male sexual performance caused by Methoprene application, the addition of protein in the diet, and the interaction of methoprene and protein. This was true whether methoprene was applied or not, although the addition of methoprene with protein resulted in the highest levels of male sexual success. Protein diet might also influence success in agonistic encounters, a frequent occurrence in lekking.
Incorporation of protein in adult diet improved male sexual success also in several other insects.

Applicant's summary and conclusion

Conclusions:

The substance incorporated into diet was not causing any adverse effects in tested organisms, in fact it improved sexual performance.