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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report date:
1999

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Qualifier:
according to guideline
Guideline:
EPA OPPTS 850.1010 (Aquatic Invertebrate Acute Toxicity Test, Freshwater Daphnids)
Qualifier:
according to guideline
Guideline:
ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))
Qualifier:
according to guideline
Guideline:
other: International Standard ISO/DIS 10706 (Water quality - Determination of long term toxicity of substances to Daphnia magna STRAUS)
GLP compliance:
yes (incl. QA statement)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- name of test material (as cited in study report): DMAC Trocken Fluessig
- chemical name: 1-Propanaminium, 3-hydroxy-N-(2-hydroxyethyl)-N, N-dimethyl-, chloride
- molecular formular: C7 H18 NO2 Cl
- batch no.: P 55
- product no.: 097852
- purity (%): 98.9 (see Analytical Report ZAX No.: 98L00661)
- molecular weight (g/mol): 183.7
- aggregated state: solid
- water-solubility: miscible
- instability against: heat
- homogeneity: yes

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- concentration control analysis were performed with test solutions of the following nominal concentrations (mg/L) of the test substance: control,
100, 12.5
- at start of test samples from vessels without daphnids, at the end of the test (after 48h) samples from vessels with and without daphnids were
analyzed

Test solutions

Vehicle:
no
Details on test solutions:
- test substance was stirred in M4 medium for ca. 10 min at 20 +/- 2 °C

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
- Test organism: Clone of Daphnia magna STRAUS 1820 supplied by Institut National de Recherche Chimique Appliquée, France, in 1978.
Since 1978, clone was bred and cultured at BASF's Laboratory of Ecology in Ludwigshafen.
- Culturing of organism: M4 medium after ISO 10706

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
2.44 mmol/L
Test temperature:
21 - 21.2°C
pH:
8 - 8.1
Dissolved oxygen:
8.7 - 9 (mg/L)
Nominal and measured concentrations:
- concentrations (mg/L): 12.5, 25, 50, 100 (nominal)
- the analytical recovery rate was > 80 % throughout the study
Details on test conditions:
- Test medium: M4
- Properties of M4 medium: hardness: 2.2 - 3.2 mmol/L
alkalinity up to pH 4.3: 0.8 - 1 mmol/L
molar ratio Ca:Mg: ca. 4:1
pH value: 7.5 - 8.5
conductivity: 550 - 650 µS/cm
- Test system:
- test vessel: test tubes (glass) with flat bottom (nominal volume 20 ml)
- test volume: 10 ml
- animals/ml: 0.5
- number of animals/vessel: 5
- number of animals/concentration: 20
- number of replicates: 4
- number of replicates for pH and O2 measurements: 1
- age of animals at start of test: 2 - 24h
- age of stock animals: 2 - 4 weeks
- illumination: artificial light, type warm white, day:night rhythm = 16:8h
- light intensity: ca. 2 - 7 µE/(m2*s) at a wave length of 400 - 700 nm
- check of the study and recording (swimming ability) visually after 0, 3, 6, 24 and 48h
- feeding: none
- measurement of temperature: continuously during whole test period in separate vessel
- measurement of pH and oxygen: after 0h in additional replicate without daphnids and after 48h in replicate 1 of each tested concentration

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC0
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
duration: 24h EC0 >= 100 mg/L nominal test mat. mobility
duration: 24h EC50 > 100 mg/L nominal test mat. mobility
duration: 24h EC100 > 100 mg/L nominal test mat. mobility

Any other information on results incl. tables

Analytically determined concentrations of the test substance in the test solutions. Values are given in percent of nominal concentrations:

Time Daphnia Values in % of nominal concentration
[h] Minimum Maximum
-----------------------------------------------------------
0 no 97.6 101.5
48 no 98.4 100.5
48 yes 101.6 101.8

The given EC-values are based on nominal test concentrations.

Applicant's summary and conclusion