1,4-bis(butylamino)anthraquinone

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1971

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: early sudy, no GLP, outdated methodology, limited report

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1971

Report date:

1971

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Deviations:

yes

Remarks:

only 1 hour exposure

GLP compliance:

no

Remarks:

pre-dates GLP regulation

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1,4-bis(butylamino)anthraquinone

EC Number:

241-379-4

EC Name:

1,4-bis(butylamino)anthraquinone

Cas Number:

17354-14-2

Molecular formula:

C22H26N2O2

IUPAC Name:

1,4-bis(butylamino)anthraquinone

Details on test material:

technically pure

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: MUS-RATTUS AG, Brunnthal, Germany
- Age at study initiation: no data
- Weight at study initiation: male: ca 315 g; female: ca. 217 g
- Fasting period before study: no data

- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: no data

ENVIRONMENTAL CONDITIONS
no data

Administration / exposure

Route of administration:

inhalation: dust

Type of inhalation exposure:

whole body

Vehicle:

air

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Source and rate of air: ambient air at 1 m³/h
- Method of conditioning air: no data
- System of generating particulates/aerosols: according to B. M. Wright (J.Scient. lnstrum. 27, 12 (1950): "A New Dust Feed Mechanism")
- Method of particle size determination: none
- Treatment of exhaust air: no data
- Temperature, humidity, pressure in air chamber: no data

TEST ATMOSPHERE
- Brief description of analytical method used: no data, concentrations were calculated
- Samples taken from breathing zone: no data

VEHICLE
- Composition of vehicle (if applicable): ambient air
- Concentration of test material in vehicle (if applicable): ca 4 mg/L

TEST ATMOSPHERE (if not tabulated)
- Particle size distribution: no data

Analytical verification of test atmosphere concentrations:

not specified

Duration of exposure:

ca. 1 h

Concentrations:

calculated as ca. 4 mg/L

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: no data
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

>= 4 mg/L air (nominal)

Based on:

test mat.

Exp. duration:

1 h

Mortality:

none

Clinical signs:

other: none

Body weight:

no data

Gross pathology:

no adverse findings

Other findings:

none

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The minimum lethal concentration after 1 hour of whole body inhalation was above 4 mg/kb bw.

Executive summary:

The inhalation toxicity of the test material was determined in rats. Animals were exposed for 1 hour to a concentration of ca. 4 mg/L (whole body, technical maximum concentration). No adverse effects on general condition nor mortality were observed. At necropsy no effects of the test material were detected.

The mean lethal dose was above 4 mg/L in rats.