2,3,4-trichlorobut-1-ene

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well-documented publication meeting basic scientific principles

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1985

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Deviations:

yes

Remarks:

only 4 animals per dose, only 15 min. exposed instead of 4 hours,

Principles of method if other than guideline:

The sensory irritation potential of several nasal tumours inducing chemicals were tested in an acute inhalation exposure experiment with rats.

GLP compliance:

not specified

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,3,4-trichlorobut-1-ene

EC Number:

219-397-9

EC Name:

2,3,4-trichlorobut-1-ene

Cas Number:

2431-50-7

Molecular formula:

C4H5Cl3

IUPAC Name:

2,3,4-trichlorobut-1-ene

Details on test material:

- Name of test material (as cited in study report): 2,3,4-Trichloro-1-butene
- Analytical purity: > 99%
- Other: Source: E.I. Du Pont, Wilmington, DE, USA.

Test animals

Species:

rat

Strain:

other: Crl-CD

Sex:

not specified

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Charles River Breeding Laboratory, North Wilmington, DE, USA
- Age at study initiation: 8 weeks
- Weight at study initiation: 232-277 g
- Fasting period before study: no data
- Housing: in pairs in stainless-steel wire-mesh cages
- Diet (e.g. ad libitum): Purina Certified Rodent Chow no. 5002, Ralston Purina, St. Louis, MO, USA)
- Water (e.g. ad libitum): yes
- Acclimation period: 7 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): no data
- Humidity (%): no data
- Air changes (per hr): no data
- Photoperiod (hrs dark / hrs light): no data

IN-LIFE DATES: :no data

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

head only

Vehicle:

other: unchanged (no vehicle)

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: 23 L rectangular polymethylmethacrylate chamber for four animals
- Exposure chamber volume: 1.5 L inner compartment for the head-only exposure of 1 animal. Airflow to the inner compartment was turned off, the
compartment was opened to the larger chamber and the 15-min test exposure began. This design enabled the rats to be essentially instantaneously exposed to the equilibrated atmosphere (initial air concentration when inner compartment is opened ts 98.3% of desired).
- Method of holding animals in test chamber: restrainer
- System of generating vapour: Atmosphere was generated by directing a stream of dry nitrogen through an impinger containing 5 to 50 ml of the liquid. The resultant vapours were diluted with houseline air prior to entry into the exposure chamber.

TEST ATMOSPHERE
- Brief description of analytical method used:
- Samples taken from breathing zone: yes
Atmospheric samples were collected every 2-3 min using gas-tight syringes. These samples were analysed on a Hewlett-Packard Model 5710A gas chromatograph (Palo Alto, CA) equipped with a flame-ionization detector. The column used was a 6 ft by 0.08 in. (ID) glass coil packed with 10% SE30 on 80/100 mesh Chromosorb® W. The test substance was chromatographed at 200°C.

TEST ATMOSPHERE (if not tabulated)
- Particle size distribution: not applicable
- MMAD (Mass median aerodynamic diameter) / GSD (Geometric st. dev.): not applicable

CLASS METHOD (if applicable)
- Rationale for the selection of the starting concentration:
1.5 ppm (6 hr/day, 5 days/wk) produced nasal tumours in rats (see 7.7, 4, Reuzel, 1981, rat, IUC 4)

Analytical verification of test atmosphere concentrations:

yes

Remarks:

GC with flame-ionization detector

Duration of exposure:

15 min

Concentrations:

0.161, 0.298, 0.380, 0.624 mg/L 
(24.3, 45.2, 57.6, 94.3 ppm)

No. of animals per sex per dose:

4 animals of unspecified sex were used per dose.

Control animals:

no

Details on study design:

- Duration of observation period following administration: 24 hours
The respiratory rate was measured during the exposure time of 15 minutes and during recovery of 5 minutes.
The body weight was measured 24 hours after exposure.

Results and discussion

Effect levels

Effect levels 1

Sex:

not specified

Dose descriptor:

other: RD50 (concentration needed to produce a 50% respiratory rate depression)

Effect level:

0.38 mg/L air

Remarks on result:

other: (58.1 ppm). For comparison: Lowest dose producing nasal tumours: 1.5 ppm

Effect levels 2

Sex:

not specified

Dose descriptor:

LC50

Effect level:

> 0.624 mg/L air

Exp. duration:

15 min

Remarks on result:

other: (94.3 ppm) Lacrimation, clear nasal discharge and salivation observed

Effect levels 3

Sex:

not specified

Dose descriptor:

other:

Effect level:

<= 0.38 mg/L air

Exp. duration:

15 min

Remarks on result:

other: (57.6 ppm). No clinical signs of systemic toxicity observed.

Mortality:

not observed

Clinical signs:

other: Lacrimation, clear nasal discharge and salivation occurred only at the highest dose of 0.624 mg/L (94.3 ppm). No clinical signs of toxicity were seen at 0.161, 0.298 and 0.380 mg/L.

Other findings:

The RD50-value (concentration needed to produce a 50% respiratory rate depression) was 0.384 mg/L (58.1 ppm);  Depression in respiratory rate occurred at all levels: The average % decrease in respiratory rate was 28, 43, 54 and 69 % at 0.161, 0.298, 0.380 mg/L and 0.624 mg/L test substance concentration.