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EC number: 201-174-2
CAS number: 79-07-2
the Klimisch 1 GLP study from Scheerbaum (2001) the growth of
Scenedesmus subspicatus was determined in the presence of Chloracetamide.For
the test interval from 0 -72 hours, the validity cirteria from OECD 201
(2006) were not met. The data were re-evaluated to meet the criteria
outlined in the updated guideline from 2006. A new evaluation after 48
hours was carried out according to OECD-Guideline No. 201 (2006) and
added as amendment to final report. The 48 hours exposure period meets
the validity criteria of the guideline for the time interval from 0 - 48
hours. This scenario is foreseen in the guideline from 2006 (Page 2,
section 11 of the guideline states: "The test period may be shortened to
at least 48 hours to maintain unlimited, exponential growth during the
test as long as the minimum multiplication factor of 16 is reached.")
Since the validity criteria were fulfilled, the results are considered
reliable. Hence, the 48 hour results as provided in the amendment of the
final report were reported here.
test was performed with Scenedesmus subspicatus (new name: Desmodesmus
subspicatus) at a control and nominal 0.313, 0.625, 1.25, 2.5, 5 and 10
mg test item/L. Six control replicates and three replicates were set up
for each treated test group. The cell density was measured via
Chlorophyll-a-fluorescence (excitation at 435 nm, emission at 685 nm).
The 48 hour-EC10 and EC50 values based on nominal concentrations were
1.67 and 4.81 mg/L, respectively.
results are considered relevant and reliable for the risk assessment.
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