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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Study period:
29 Feb - 02 Mar 2007
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method C.3 (Algal Inhibition test)
Deviations:
not specified
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Water Accomodated Fraction (WAF): A concentration of 100 mg/L of the test substance was moderately stirred in algal medium for 24 h at room temperature. After this incubation time, the undissolved materials were removed by sterile filtration (0.45 µm) to obtain a sterile test substance solution for the algal growth test. The resulting water accommodated fraction was used in the test.
Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM
- Strain: 86.81 SAG
- Source: obtained from the collection of Algal Cultures, Institute of Freshwater Ecology, University of Göttingen, Göttingen, Germany
- Age of inoculum (at test initiation): Exponentially growing liquid preculture were used to inoculate sterile test flasks.
- Method of cultivation: 250 mL flask containing 100 mL of sterile OECD medium inoculated with cell material from axenic slope culture, continuous illumination from warm white fluorescent tubes (8500 - 9000 lux), temperature: 22 ± 0.2 °C thermocontrolled room, periodically conducted reference test with potassium dichromate.
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h
Hardness:
Not specified
Test temperature:
22 ± 0.2 °C
pH:
8.0 ± 0.2
Dissolved oxygen:
Not specified
Salinity:
Not specified
Conductivity:
Not specified
Nominal and measured concentrations:
Nominal: 100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Erlenmeyer flask
- Material: glass; Size: 250 mL; Fill volume: 100 mL
- Initial cells density: 10000 - 20000/mL
- No. of vessels per concentration: 3
- No. of vessels per control: 5

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: OECD medium
- Culture medium different from test medium: no
- Intervals of water quality measurement: Temperature was determined in a control flask at the start and at the end of the test. The pH was determined in 1 control flask after sterilization before the test and in all test vessels at the end of the test. Observations of mis-shapen cells and cell-debris were made after 24, 48 and 72 h test duration.

OTHER TEST CONDITIONS
- Photoperiod: continuous
- Light intensity and quality: warm white fluorescent tubes (8500-9000 lux)

EFFECT PARAMETERS MEASURED: Algal growth was recorded after 24, 48 and 72 h test duration.
- Determination of cell concentrations: using a spectrophotometer (680 nm wavelength). Aliquots of 5 mL were removed from each test substance served as controls. The starting cell density was determined using a counting chamber.
Reference substance (positive control):
no
Key result
Duration:
72 h
Dose descriptor:
EL50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: EL50 (24-72h)
Key result
Duration:
72 h
Dose descriptor:
EL0
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: EL0 (24-72h)
Key result
Duration:
72 h
Dose descriptor:
EL50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Remarks on result:
other: EL50 (24-72h)
Key result
Duration:
72 h
Dose descriptor:
EL0
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Remarks on result:
other: EL0 (24-72h)
Details on results:
- Exponential growth in the control: yes
- Observation of abnormalities: no

At the WAF loading rate of 100 mg/L no inhibition with respect to algal growth rate and algal cell growth (area under growth curve) compared to the untreated controls were observed. The no-observed-effect concentration for growth rates (ErL0) and biomass production (EbL0) with respect to the loading rate was ≥ 100 mg/L. The EL50 -value was conducted as EL50 (24 -72 h). But the EL50 can also be stated as EL50 (72 h) > 100 mg/L for the algal growth rate and algal cell growth.

Table 1: Cell concentration (A680) in individual test cultures after 24, 48 and 72 h of exposure to the test material. ((b) = Mean absorbance (A680))

Nominal concentration [mg/L]

Code

Cell concentration (A680)

0 h (b)

24 h

48 h

72 h

Control

A

B

C

D

E

0.008

0.008

0.008

0.008

0.008

0.033

0.038

0.038

0.036

0.040

0.228

0.222

0.208

0.212

0.233

0.685

0.733

0.676

0.624

0.694

100

A

B

C

0.008

0.008

0.008

0.040

0.038

0.044

0.224

0.207

0.207

0.700

0.631

0.675

 

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Study period:
03 Sep - 29 Sep 2012
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
GLP - Guideline study, tested with the source substance Heptanoic acid, ester with 2,2-dimethyl-1,3-propanediol (CAS No. 68855-18-5). According to the ECHA guidance document “Practical guide 6: How to report read-across and categories (March 2010)”, the reliability was changed from RL1 to RL2 to reflect the fact that this study was conducted on a read-across substance.
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
not specified
Qualifier:
according to guideline
Guideline:
EU Method C.3 (Algal Inhibition test)
Deviations:
not specified
GLP compliance:
yes (incl. QA statement)
Remarks:
The Department of Health of the Government of the United Kingdom, date of inspection: 10 July 2012
Analytical monitoring:
yes
Details on sampling:
- Sampling method: Samples were taken from the solvent control and the 100% v/v test group (replicates R1 – R6 pooled) at 0 and 72 hours for quantitative analysis.
- Sample storage conditions before analysis: All 0-Hour samples were stored at approximately -20 °C prior to analysis. Duplicate samples were taken at 0 and 72 hours and stored at approximately -20 ºC for further analysis if necessary.
Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Based on the result of the range-finding test a "limit test" was conducted at a concentration of 100% v/v to confirm that at the highest attainable concentration, no effect on algal growth was observed. For the purpose of the definitive test, the test item was prepared using a preliminary solution in dimethylformamide. An amount of test item (100 mg) was dissolved in dimethylformamide and the volume adjusted to 10 mL to give a 100 mg/10 mL solvent stock solution. An aliquot (200 µL) of this solvent stock solution was dispersed in 2 liters of culture medium with the aid of magnetic stirring for approximately 10 minutes. After stirring any undissolved test item was removed by filtration through a 0.2 µm Gelman Acrocap filter (first approximate 500 mL discarded in order to pre-condition the filter) to give a 100% v/v solution of test item. An aliquot (1 liter) of the stock solution was inoculated with algal suspension (10.9 mL) to give the required test concentration of 100% v/v (equivalent to a 0-Hour measured test concentration of 0.018 mg/L). The stock solutions and the prepared concentration were inverted several times to ensure adequate mixing and homogeneity.
The concentration and stability of the test item in the test preparations were verified by chemical analysis at 0 and 72 hours.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): dimethylformamide
- Evidence of undissolved material (e.g. precipitate, surface film, etc): At the start of the test all control, solvent control and test cultures were observed to be clear colorless solutions. After the 72-Hour test period all control, solvent control and test cultures were observed to be pale green dispersions.
Test organisms (species):
Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
Details on test organisms:
TEST ORGANISM
- Common name: green alga
- Strain: CCAP 278/4
- Source (laboratory, culture collection): Obtained from the Culture Collection of Algae and Protozoa (CCAP), SAMS Research Services Ltd, Scottish Marine Institute, Oban, Argyll, Scotland.
- Method of cultivation: Master cultures were maintained in the laboratory by the periodic replenishment of culture medium. The master cultures were maintained in the laboratory under constant aeration and constant illumination at 21 ± 1 °C.
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h
Hardness:
Not specified
Test temperature:
24 ± 1 °C
pH:
7.5 - 7.9
Dissolved oxygen:
Not specified
Salinity:
Not specified
Conductivity:
Not specified
Nominal and measured concentrations:
100% v/v (nominal)
0.018 mg/L (measured)
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 250 mL glass conical flasks
- Initial cells density: 5.52 x 10^3 cells/mL (mean)
- Control end cells density: 8.11 x 10^5 cells/mL (mean)
- Mean cell density of solvent control at 0 hours: 5.51 x 10^3 cells/mL
- Mean cell density of solvent control at 72 hours: 1.00 x 10^6 cells/mL
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates): 6

GROWTH MEDIUM
- Standard medium used: yes, according to OECD guideline 201

OTHER TEST CONDITIONS
- Adjustment of pH: yes, adjusted to 7.5 ± 0.1 with 0.1N NaOH or HCl
- Photoperiod: continuous illumination
- Light intensity and quality: 7000 lux, provided by warm white lighting (380 – 730 nm)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Coulter Multisizer Particle Counter

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 10 an 100% v/v (nominal)
- Results used to determine the conditions for the definitive study: The results showed no effect on growth at the test concentrations of 10 and 100% v/v.
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Key result
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
> 0.006 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
other: Test mat. WAF
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 0.006 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
other: Test mat. WAF
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.006 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
other: Test mat. WAF
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
> 0.006 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
other: Test mat. WAF
Basis for effect:
other: yield
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 0.006 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
other: Test mat. WAF
Basis for effect:
other: yield
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.006 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
other: Test mat. WAF
Basis for effect:
other: yield
Details on results:
- Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): no
Results with reference substance (positive control):
Exposure of Pseudokirchneriella subcapitata (CCAP 278/4) to the reference item gave the following results:
ErC50 (0 – 72 h): 1.1 mg/L; 95% confidence limits 1.0 – 1.3 mg/L
EyC50 (0 – 72 h): 0.70 mg/L
NOEC) based on growth rate: 0.50 mg/L
NOEC) based on yield: 0.25 mg/L
LOEC) based on growth rate: 1.0 mg/L
LOEC) based on yield: 0.50 mg/L
Reported statistics and error estimates:
A Student’s t-test incorporating Bartlett's test for homogeneity of variance (Sokal and Rohlf, 1981, Biometry. New York: W H Freeman and Company) was carried out on the growth rate and yield data after 72 hours for the solvent control and the 100% v/v test concentration to determine any statistically significant differences between the test and solvent control groups. All statistical analyses were performed using the SAS computer software package (SAS, 1999 - 2001, SAS/STAT Proprietary Software Release 8.02, SAS Institute Inc, Cary, NC, USA).

Range-finding Test:

The results showed no effect on growth at the test concentrations of 10 and 100% v/v. Based on this information a single test concentration of six replicates, of 100% v/v was selected for the definitive test. This experimental design conforms to a "limit test" to confirm that at the highest attainable test concentration, no effect on growth was observed. Chemical analysis of the 100% v/v test preparation at 0 hours showed a measured test concentration of 0.029 mg/L was obtained. A significant decline in measured test concentration was observed at 72 hours to less than the limit of quantitation (LOQ) of the analytical method employed which was determined to be 0.0045 mg/L. These results indicated that the test item was unstable over the test duration.

Table: Cell Densities and pH Values in the Definitive Test

Geometric Mean Measured Test Concentration (mg/L)

pH

Cell densities (cells/mL) (mean number of cells per mL calculated from the mean of the cell counts from 3 counts for each of the replicate flasks)

pH

0 h

0 h

24 h

48 h

72 h

72 h

Control

R1

R2

R3

R4

R5

R6

mean

7.5

4.93E+03

2.85E+04

1.66E+05

8.08E+05

7.9

5.17E+03

2.98E+04

1.75E+05

8.18E+05

5.57E+03

3.19E+04

1.77E+05

9.25E+05

5.92E+03

2.76E+04

1.47E+05

7.29E+05

5.78E+03

3.09E+04

1.41E+05

8.16E+05

5.76E+03

2.59E+04

1.50E+05

7.69E+05

5.52E+03

2.91E+04

1.59E+05

8.11E+05

Solvent control

R1

R2

R3

R4

R5

R6

mean

7.5

5.50E+03

3.64E+04

2.20E+05

1.16E+06

7.9

5.42E+03

3.22E+04

1.70E+05

9.84E+05

5.68E+03

2.68E+04

1.54E+05

7.72E+05

5.18E+03

3.23E+04

1.94E+05

1.11E+06

5.46E+03

2.77E+04

1.65E+05

9.21E+05

5.79E+03

2.76E+04

2.04E+05

1.07E+06

5.51E+03

3.05E+04

1.84E+05

1.00E+06

0.0065

R1

R2

R3

R4

R5

R6

mean

7.5

5.84E+03

4.32E+04

2.93E+05

1.44E+06

7.9

5.70E+03

2.92E+04

1.73E+05

1.02E+06

4.67E+03

2.77E+04

1.91E+05

1.04E+06

5.31E+03

2.95E+04

1.85E+05

1.41E+06

5.42E+03

3.02E+04

1.79E+05

9.67E+05

5.22E+03

2.52E+04

1.79E+05

9.35E+05

5.36E+03

3.08E+04

2.00E+05

1.13E+06

Table: Inhibition of Growth Rate and Yield in the Definitive Test

Geometric Mean Measured Test Concentration (mg/L)

Growth Rate (cells/mL/hour)

Yield (cells/mL)

0 – 72 h

% Inhibition

0 – 72 h

% Inhibition

Control

R1

R2

R3

R4

R5

R6

Mean

SD

0.071

-

8.03E+05

-

0.071

8.13E+05

0.073

9.20E+05

0.069

7.23E+05

0.071

8.11E+05

0.070

7.63E+05

0.071

8.06E+05

0.001

6.59E+04

Solvent control

R1

R2

R3

R4

R5

R6

Mean

SD

0.076

-

1.16E+06

-

0.073

9.78E+05

0.070

7.67E+05

0.075

1.11E+06

0.072

9.15E+05

0.075

1.07E+06

0.074

9.99E+05

0.002

1.43E+05

0.0065

R1

R2

R3

R4

R5

R6

Mean

SD

0.079

(7)

1.43E+06

 

0.074

0

1.01E+06

 

0.074

0

1.04E+06

 

0.078

(5)

1.40E+06

 

0.073

1

9.61E+05

 

0.073

1

9.30E+05

 

0.075

(2)

1.13E+06

(13)

0.003

 

2.27E+05

 
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Study period:
12 - 15 Dec 2006
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions (no purity, no analytical monitoring)
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method C.3 (Algal Inhibition test)
Deviations:
not specified
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Water Accomodated Fraction (WAF): The test substance (100 mg/L) was moderately stirred in algal medium for 24 h at room temperature. After this incubation time, the undissolved materials were removed by sterile filtration (0.45 µm) to obtain a sterile test substance solution. The resulting WAF was used in the test.
- Controls: yes water control
Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
TEST ORGANISM
- Common name: green algae
- Strain: 86.81 SAG
- Source (laboratory, culture collection): Collection of Algal Cultures, Institute of Freshwater Ecology, University of Göttingen, Germany
- Method of cultivation: Cultivated in 250 mL flasks containing 100 mL of sterile OECD medium inoculated with cell material from axenic slope culture at continuous illumination from warm white fluorescent tubes (8500-9000 lux) and 22 ± 0.2 °C (thermocontrolled room).
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h
Hardness:
Not specified
Test temperature:
22 ± 0.2 °C
pH:
at the beginning of incubation: 8.0 ± 0.2
Dissolved oxygen:
Not specified
Salinity:
Not specified
Conductivity:
Not specified
Nominal and measured concentrations:
Nominal: 0 and 100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 250 mL flaks, all-glass, with 100 mL of test medium
- Initial cells density: 10000- 20000 / mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 5
- Other: Test flasks were placed on an orbital shaker at a nominal shaking speed of 140 RPM.

GROWTH MEDIUM
- Standard medium used: yes, OECD medium

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: OECD medium
- Culture medium different from test medium: no
- Intervals of water quality measurement: Temperature was determined in a control flask at the start and at the end of the test. pH was determined in 1 control flask after sterilisation before the test and in all test vessels at the end of the test.

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: Continuous
- Light intensity and quality: From warm white fluorescent tubes (8500-9000 lux)

EFFECT PARAMETERS MEASURED: Determination of cell concentration and observations of mis-shapen cells and cell-debris were made after 24, 48 and 72 h of exposure.
- Determination of cell concentrations: Cell concentration was determined every 24 h using a spectrophotometer (680 nm wavelength). Aliquots of 5 mL were removed from each test substance served as controls. The starting cell density was determined using a counting chamber.
Reference substance (positive control):
yes
Remarks:
potassium dichromate
Key result
Duration:
72 h
Dose descriptor:
EL50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
WAF
Basis for effect:
growth rate
Remarks on result:
other: EL50 (24 - 72 h)
Key result
Duration:
72 h
Dose descriptor:
EL50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
WAF
Basis for effect:
biomass
Remarks on result:
other: EL50 (24 - 72 h)
Key result
Duration:
72 h
Dose descriptor:
EL0
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
WAF
Basis for effect:
growth rate
Remarks on result:
other: EL0 (24 - 72 h)
Key result
Duration:
72 h
Dose descriptor:
EL0
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Remarks:
WAF
Basis for effect:
biomass
Remarks on result:
other: EL0 (24 - 72 h)
Details on results:
- Exponential growth in the control (for algal test): yes
- Any stimulation of growth found in any treatment: 1.1% stimulation of growth rates at 100 mg/L; 20.9% stimulation of biomass at 100 mg/L

Table 1: Cell concentration (A680) in individual test cultures after 24, 48 and 72 h of exposure to the test material ((b) = Mean absorbance (A680))

Nominal concentration [mg/l]

Code

Cell concentration (A680)

0 h (b)

24 h

48 h

72 h

Control

A

B

C

D

E

0.005

0.005

0.005

0.005

0.005

0.019

0.020

0.017

0.017

0.018

0.116

0.150

0.122

0.137

0.131

0.549

0.667

0.543

0.556

0.573

100

A

B

C

0.005

0.005

0.005

0.023

0.021

0.021

0.149

0.111

0.189

0.776

0.649

0.720

 

Table 2: Average specific growth rates between 24 and 72 h

Nominal concentration [mg/l]

Code

Specific growth rate (x 0.01)

Coefficient of correlation (R)

Inhibition (%)

Mean value (%)

Standard deviation (%)

Control

A

B

C

D

E

7.01

7.31

7.22

7.27

7.21

0.999

0.996

0.997

0.994

0.996

2.7

-1.5

-0.2

-0.9

-0.1

0.0

1.6

100

A

B

C

7.33

7.15

7.36

0.999

1.000

0.990

-1.8

0.7

-2.3

-1.1

1.6

 

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study conducted in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do not affect the quality of the relevant results.
Justification for type of information:
Please see attached justification for read across in Section 13.
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 0.006 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
other: Test mat. WAF
Basis for effect:
growth rate
Remarks on result:
other: CAS 68855-18-5
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.006 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
other: Test mat. WAF
Basis for effect:
growth rate
Remarks on result:
other: CAS 68855-18-5
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 0.006 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
other: Test mat. WAF
Basis for effect:
other: yield
Remarks on result:
other: CAS 68855-18-5
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
0.006 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
other: Test mat. WAF
Basis for effect:
other: yield
Remarks on result:
other: CAS 68855-18-5
Key result
Duration:
72 h
Dose descriptor:
EL50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: CAS 11138-60-6
Key result
Duration:
72 h
Dose descriptor:
EL50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
other: test mat. /WAV
Basis for effect:
growth rate
Remarks on result:
other: CAS 85186-86-3
Key result
Duration:
72 h
Dose descriptor:
EL50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
other: test mat. /WAV
Basis for effect:
biomass
Remarks on result:
other: CAS 85186-86-3

Description of key information

No effects up to the limit of water solubility for Desmodesmus subspicatus (OECD 201); based on read-across from supporting substance (structural analogue or surrogate).

Key value for chemical safety assessment

EC50 for freshwater algae:
0.006 mg/L
EC10 or NOEC for freshwater algae:
0.006 mg/L

Additional information