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EC number: 816-315-9 | CAS number: 70024-75-8
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions (purity not reported, no GLP data)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- not specified
- GLP compliance:
- not specified
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: Station Epuration Wavre 2nd Stage, 23 Feb 1999
- Concentration of inoculum: > 1000000 CFU/mL (verification with Sanicheck) (CFU = Colony Forming Unit) - Duration of test (contact time):
- 28 d
- Initial conc.:
- 20 mg/L
- Based on:
- other: TOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Clearly chemically defined medium
- Test temperature: 22.1 - 22.4 °C
- pH: 7.2 - 7.3
- Aeration of dilution water: 24 h with CO2-free air (50 - 100 mL/min)
TEST SYSTEM
- Culturing apparatus: Container flask with 3 L of solution test and 30 mL inoculum
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: CO2-free air
- Details of trap for CO2 and volatile organics if used: The produced CO2 reacts with Ba(OH)2 to give a precipitate of Barium carbonate.
SAMPLING
- Sampling frequency: Sampling was performed on day 0, 2, 6, 9, 15, 19, 23 and 28.
- Sampling method: The produced CO2 was determinated in titrating the remaining Ba(OH)2 with HCl and phenolphtaleine.
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 3 g/L
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 93.1
- Sampling time:
- 28 d
- Details on results:
- Within the 10-day time window the 60% level was passed. Therefore, the test substance is regarded as readily biodegradable.
- Results with reference substance:
- Degradation: 77.7% after 9 days, 60% pass level was reached.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- 12 Sep - 10 Oct 2006
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions (purity not reported)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Deviations:
- not specified
- GLP compliance:
- not specified
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Activated sludge from the aeration tank of a municipal waste water treatment plant, ARA Werdhölzli,
Zürich, Switzerland (06 Sep 2006)
- Pretreatment: The sludge was washed twice with tap water. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 22.6 mg/L
- Based on:
- other: TOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Aerobic mineral salts medium prepared with deionised water
- Test temperature: 22 ± 0.5 °C
- pH adjusted: Yes, the pH-value was checked at the beginning and at the end of the test and adjusted to pH 7.4 (± 0.2) with NaOH or HCL.
- Suspended solids concentration: 30 mg/L
- Continuous darkness: Yes, temperature-controlled dark room
TEST SYSTEM
- Culturing apparatus: 2500 mL closed glass bottle containing a total volume of test solution of 2000 mL
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: Aerated with synthetic CO2-free air for a maximum test period of 28 days.
- Measuring equipment: Shimadzu 5050 TOC-Analyzer using the NPOC-mode for DOC-determination
- Details of trap for CO2: The air leaving the individual vessels was passed through gas-absorption bottles filled with NaOH. The trapped CO2 was determined as inorganic carbon (IC).
SAMPLING
- Sampling frequency: on Day 0, 1, 3, 7, 10, 14, 17, 21, 24 and 28
- Sampling method: Samples were centrifuged (15 min at 4500 g) and acidified to pH < 2. Prior to analysis the samples were sparged with CO2-free
high purity air for 10 min to remove inorganic carbon.
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, 2 replicates - Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 20.3 mg/L (TOC)
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 90.2
- Sampling time:
- 28 d
- Details on results:
- Within the 10-day time window the 60% level was passed. Therefore, the test substance is regarded as readily biodegradable.
- Results with reference substance:
- Degradation: 83.1% after 14 days, 60% pass level was reached.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do not affect the quality of the relevant results.
- Justification for type of information:
- Please see attached justification for read across in Section 13.
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- GLP compliance:
- not specified
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- >= 90.2 - <= 93.1
- Sampling time:
- 28 d
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
Referenceopen allclose all
Table 1: Biodegradation of test (mean of 2 replicates) and reference substance
Day |
Reference |
Test substance |
||
% CO2 produced |
%CO2 total |
% CO2 produced |
%CO2 total |
|
0 |
0.0 |
0.0 |
0.0 |
0.0 |
2 |
40.5 |
40.5 |
14.0 |
21.0 |
6 |
28.7 |
69.1 |
15.7 |
44.5 |
9 |
8.6 |
77.7 |
15.4 |
67.6 |
15 |
3.9 |
81.7 |
12.4 |
86.2 |
19 |
5.1 |
86.8 |
2.8 |
90.3 |
23 |
3.1 |
89.9 |
1.2 |
92.1 |
28 |
1.2 |
91.1 |
0.7 |
93.1 |
Table 2: Biodegradation of the replicates of the test substance
Day |
Test substance 1 |
Test substance 2 |
||
% CO2 produced |
%CO2 total |
% CO2 produced |
%CO2 total |
|
0 |
0.0 |
0.0 |
0.0 |
0.0 |
2 |
20.7 |
20.7 |
21.3 |
21.3 |
6 |
19.8 |
40.5 |
27.2 |
48.5 |
9 |
26.8 |
67.3 |
19.3 |
67.8 |
15 |
17.2 |
84.5 |
20.0 |
87.8 |
19 |
4.5 |
89.0 |
3.9 |
91.7 |
23 |
1.7 |
90.7 |
1.9 |
93.5 |
28 |
1.0 |
91.7 |
1.0 |
94.5 |
The biodegradation of the test substance reached 74% at the end of the 10-d window.
Table 1: Biodegradation of test substance
Time (days) |
Test substance 1 |
Test substance 2 |
|
||
Total CO2 release in test sample (mg IC/L) |
Degradation (%) |
Total CO2 release in test sample (mg IC/L) |
Degradation (%) |
Mean degradation (%) |
|
0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
1 |
0.0 |
0.0 |
-0.1 |
-0.1 |
0.0 |
3 |
1.7 |
4.3 |
2.0 |
5.7 |
5.0 |
7 |
10.3 |
37.0 |
8.7 |
30.2 |
33.6 |
10 |
16.0 |
58.4 |
15.3 |
55.4 |
56.9 |
14 |
19.9 |
75.5 |
20.0 |
75.9 |
75.7 |
17 |
21.1 |
81.4 |
21.3 |
82.4 |
81.9 |
21 |
23.0 |
86.4 |
22.8 |
85.6 |
86.0 |
24 |
23.1 |
86.8 |
24.0 |
90.6 |
88.7 |
28 |
23.7 |
88.1 |
24.6 |
92.2 |
90.2 |
Table 2: Biodegradation of reference substance and Inoculum blank
Time (days) |
Reference substance |
Inoculum blank |
|
Total CO2 release in test sample (mg IC/L) |
Degradation (%) |
Total CO2 release in test sample (mg IC/L) |
|
0 |
0.0 |
0.0 |
0.0 |
1 |
0.6 |
3.3 |
0.0 |
3 |
7.7 |
34.3 |
0.8 |
7 |
14.2 |
60.4 |
1.9 |
10 |
17.6 |
73.0 |
2.8 |
14 |
19.7 |
83.1 |
2.8 |
17 |
20.3 |
87.1 |
2.7 |
21 |
21.6 |
89.5 |
3.5 |
24 |
21.7 |
89.8 |
3.5 |
28 |
22.3 |
91.3 |
3.8 |
Description of key information
Readily biodegradable: 93.1% (CO2 evolution) in 28 days (OECD 301 B); based on read-across from supporting substance (structural analogue or surrogate).
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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