3-[[9,10-dihydro-9,10-dioxo-4-[(p-tolyl)amino]-1-anthryl]aminopropyl]trimethylammonium methyl sulphate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

19 Jul - 17 Aug 1994

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Benzyl[3-[[9,10-dihydro-4-[isopropylamino]-9,10-dioxo-1-anthryl]amino]propyl]dimethylammonium chloride

EC Number:

252-547-1

EC Name:

Benzyl[3-[[9,10-dihydro-4-[isopropylamino]-9,10-dioxo-1-anthryl]amino]propyl]dimethylammonium chloride

Cas Number:

35402-38-1

Molecular formula:

C29H34N3O2.Cl

IUPAC Name:

N-benzyl-3-{[4-(isopropylamino)-9,10-dioxo-9,10-dihydroanthracen-1-yl]amino}-N,N-dimethylpropan-1-aminium chloride

Test material form:

solid: particulate/powder

Details on test material:

Basic Blue 123 Cl

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, predominantly domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge: ARA Reinach CH, 19 July 1994

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

25 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

TEST CONDITIONS
- Composition of medium: mineral medium according to OECD 302B
- Test temperature: Room temperature
- pH adjusted: no
- Lighting: indirect daylight

TEST SYSTEM
- Culturing apparatus: glass vessels
- Number of culture flasks/concentration: 2
- Other: total test volume 250 mL per vessel

Measurement frequency: 1, 2, 5, 6, 7, 9, 13, 16, 19, 21, 23, 26, 27 and 28 d

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: no, but OECD 209 run 9in parallel (3-h IC50 = 66 mg/L)

Reference substance

Reference substance:

diethylene glycol

Results and discussion

% Degradation

Key result

Parameter:

% degradation (DOC removal)

Value:

37.5

Sampling time:

28 d

Details on results:

Adsorption after 3 h: ca. 20.5%

BOD5 / COD results

Results with reference substance:

100% biodegradation after 6 d

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not inherently biodegradable

Conclusions:

The test item is not inherently biodegradable based on a biodegradation rate of 37.5% after 28 d (DOC removal).