methyl acrylate; methyl propenoate

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1982

Report date:

1982

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Methyl acrylate

EC Number:

202-500-6

EC Name:

Methyl acrylate

Cas Number:

96-33-3

Molecular formula:

C4H6O2

IUPAC Name:

methyl prop-2-enoate

Details on test material:

- Test substance: Methylacrylat
- Test substance No.: 82/22
- Analytical purity: ca. 99.95 %

Test animals

Species:

rabbit

Strain:

other: New Zealand, unspecified

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Gaukler, Offenbach/Main, D
- Weight at study initiation: 2.91 kg (males); 2.98 kg (females)
- Acclimation period: 8 d


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 - 26
- Humidity (%): ca. 30 - 70
- Photoperiod (hrs dark / hrs light): 12/12

Test system

Type of coverage:

semiocclusive

Preparation of test site:

shaved

Vehicle:

unchanged (no vehicle)

Controls:

other: untreated skin sites of the same animals

Amount / concentration applied:

0.5 mL

Duration of treatment / exposure:

1 or 4 h

Observation period:

7 d

Number of animals:

6 (3 per sex)

Details on study design:

TEST SITE
- Area of exposure: 3x3cm, upper third of the flank/ back region
- Type of wrap if used: Hansamed plaster, Beiersdorf AG, D


REMOVAL OF TEST SUBSTANCE
- Washing (if done): Lutrol and Lutrol/water 1:1

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

mean

Remarks:

4 h exposure

Time point:

24/48/72 h

Score:

2.17

Max. score:

3

Reversibility:

not fully reversible within: 7 d

Results 2

Irritation parameter:

edema score

Basis:

mean

Remarks:

4 h exposure

Time point:

24/48/72 h

Score:

2.44

Max. score:

3

Reversibility:

not fully reversible within: 7 d

Results 3

Irritation parameter:

erythema score

Basis:

mean

Remarks:

1 h exposure

Time point:

24/48/72 h

Score:

2.44

Max. score:

3

Reversibility:

not fully reversible within: 7 d

Results 4

Irritation parameter:

edema score

Basis:

mean

Remarks:

1 h exposure

Time point:

24/48/72 h

Score:

2.33

Max. score:

3

Reversibility:

not fully reversible within: 7 d

Any other information on results incl. tables

Exposition:	4 h semi-occlusive
Animal	Reading	Erythema	Edema	Comment
1	1 h	2	3	erythema overlapping, hemorragic; edema overlapping
2	1 h	2	3	erythema and edema overlapping
3	1 h	2	3	erythema and edema overlapping
4	1 h	2	2	erythema and edema overlapping
5	1 h	2	3	erythema overlapping, hemorragic; edema overlapping
6	1 h	3	3	erythema overlapping, hemorragic; edema overlapping
1	24 h	2	2	erythema overlapping, hemorragic; edema overlapping
2	24 h	2	3	erythema and edema overlapping
3	24 h	2	3	erythema and edema overlapping
4	24 h	2	2	erythema and edema overlapping
5	24 h	2	3	erythema overlapping, hemorragic; edema overlapping
6	24 h	3	3	erythema overlapping, hemorragic; edema overlapping
1	48 h	2	2	erythema overlapping, hemorragic; edema overlapping
2	48 h	2	3	erythema and edema overlapping
3	48 h	2	3	erythema and edema overlapping
4	48 h	2	2	erythema and edema overlapping
5	48 h	2	2	erythema and edema overlapping
6	48 h	3	2	erythema overlapping
1	72 h	2	2	erythema overlapping, hemorragic; edema overlapping
2	72 h	2	3	erythema and edema overlapping
3	72 h	2	3	erythema and edema overlapping
4	72 h	2	2	erythema and edema overlapping
5	72 h	2	2	erythema and edema overlapping
6	72 h	3	2	erythema overlapping
1	7 d	2	1	scaling
2	7 d	1	0
3	7 d	2	1
4	7 d	1	0	scaling
5	7 d	1	0	erythema overlapping, scaling
6	7 d	2	1	strong scaling, edema overlapping
mean	24 - 72 h	2.17	2.44
Exposition:	1 h semi-occlusive
Animal	Reading	Erythema	Edema	Comment
1	1 h	2	3	erythema and edema overlapping
2	1 h	2	3	erythema and edema overlapping
3	1 h	2	3	erythema and edema overlapping
4	1 h	2	2	erythema and edema overlapping
5	1 h	2	3	erythema overlapping, hemorragic; edema overlapping
6	1 h	3	3	erythema overlapping, hemorragic; edema overlapping
1	24 h	2	3	erythema and edema overlapping
2	24 h	2	3	erythema and edema overlapping
3	24 h	2	3	erythema overlapping, hemorragic; edema overlapping
4	24 h	2	2	erythema and edema overlapping
5	24 h	1	2	erythema and edema overlapping
6	24 h	3	3	erythema and edema overlapping
1	48 h	2	2	erythema and edema overlapping
2	48 h	3	3	erythema and edema overlapping
3	48 h	3	3	erythema overlapping, hemorragic; edema overlapping
4	48 h	2	1	erythema and edema overlapping
5	48 h	4	2	necrosis, edema overlapping
6	48 h	2	3	edema overlapping
1	72 h	2	2	erythema and edema overlapping
2	72 h	3	3	erythema and edema overlapping
3	72 h	3	3	erythema overlapping, hemorragic; edema overlapping
4	72 h	2	1
5	72 h	4	2	necrosis, edema overlapping
6	72 h	2	1
1	7 d	1	0	scaling
2	7 d	1	0
3	7 d	2	1	scaling, erythema and edema overlapping
4	7 d	0	0	scaling
5	7 d	4	1	superficial necrosis; edema overlapping
6	7 d	1	0	scaling, erythema overlapping
mean	24 - 72 h	2.44	2.33

Applicant's summary and conclusion

Interpretation of results:

irritating

Remarks:

Migrated information Criteria used for interpretation of results: EU