2-methoxyethyl acrylate

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions. No details on analytical purity of the test substance. Occlusive test conditions and limited details on test animals and environmental conditions.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1968

Report date:

1968

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

yes

Remarks:

no details on analytical purity of the test substance; occlusive test conditions; limited details on test animals and environmental conditions

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-methoxyethyl acrylate

EC Number:

221-499-3

EC Name:

2-methoxyethyl acrylate

Cas Number:

3121-61-7

Molecular formula:

C6H10O3

IUPAC Name:

2-methoxyethyl acrylate

Details on test material:

- Name of test material (as cited in study report): Methyl Cellosolve Acrylate, 2-Methoxyethoxy Propenoate
- Analytical purity: no data
- Other: Sample No. 31-126; Reg. No. 511-01-4353, received from Union Carbide Corporation, Chemicals and Plastics Division, South Charleston, WV

Test animals

Species:

rabbit

Strain:

not specified

Sex:

not specified

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Housing: animals were housed in immobilization devices in inhalation chambers with heads protruding into the study room to prevent inhalation of the substance. After treatment, animals were returned to their standard housing.

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

TEST SITE
- Area of exposure: clipped trunk of the rabbit under a cotton pad
- Type of wrap if used: the entire trunk was then wrapped in an impervious Vinylite covering.

REMOVAL OF TEST SUBSTANCE
- Washing (if done): residual test substance was washed.
- Time after start of exposure: 24 h

TEST MATERIAL
- Amount(s) applied: 0.125, 0.25 and 0.5 mL/kg

Duration of exposure:

24 h

Doses:

0.125 mL/kg bw corresponding to 126.25 mg/kg bw
0.25 mL/kg bw corresponding to 252.5 mg/kg bw
0.5 mL/kg bw corresponding to 505 mg/kg bw

Based on a test substance density of 1.01 g/cm³.

No. of animals per sex per dose:

4

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Statistics:

The LD50 value was calculated via the probit method.

Results and discussion

Effect levels

Effect levels 1

Sex:

not specified

Dose descriptor:

LD50

Effect level:

ca. 252.5 mg/kg bw

Based on:

test mat.

95% CL:

ca. 171.7 - ca. 373.7

Remarks on result:

other: based on a density of 1.01 g/cm³

Effect levels 2

Sex:

not specified

Dose descriptor:

LD50

Effect level:

ca. 0.25 mL/kg bw

Based on:

test mat.

95% CL:

0.17 - 0.37

Mortality:

126.25 mg/kg bw: no animal died
252.5 mg/kg bw: 2 animals died
505.0 mg/kg bw: all animals died

Clinical signs:

other: No clinical signs of toxicity were observed in the animals at any dose level.

Gross pathology:

At gross pathology, mottled liver was found.

Other findings:

- Other observations:
No skin irritation was observed in the surviving animals treated with 252.5 and 505 mg/kg bw.

Any other information on results incl. tables

Table 1. Acute dermal toxicity

Dose [mg/kg bw]	Toxicological results*	Duration of clinical signs	Time of death	Mortality (%)
Males
126.25	0/0/4	---	---	0
252.5	2/0/4	---	Day 1	50
505	4/0/4	---	Day 2	100
LD50 = 252.5 mg/kg bw

  *first number = number of dead animals

second number = number of animals with clinical signs

third number = number of animals used

Table 2. Body weight changes

Dose [mg/kg bw]	Weight Change*
126.25	+++-
252.5	+-
505	N/A

*all surviving animals;

+ = animals with weight gain

- = animals with weight loss

Applicant's summary and conclusion

Interpretation of results:

Category 3 based on GHS criteria

Conclusions:

CLP: Acute dermal 3, H311