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Environmental fate & pathways

Hydrolysis

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Administrative data

Endpoint:
hydrolysis
Type of information:
experimental study
Adequacy of study:
key study
Study period:
05 April - 09 May 1995
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study carried out in accordance with method C7 of commission directive 92/69/EEC and in accordance with GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report date:
1995

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EU Method C.7 (Degradation: Abiotic Degradation: Hydrolysis as a Function of pH)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
-
EC Number:
414-490-2
EC Name:
-
Cas Number:
154212-59-6
Molecular formula:
C11H9ClN2O5S
IUPAC Name:
4-nitrophenyl 1,3-thiazol-5-ylmethylcarbonate hydrochloride

Study design

Analytical monitoring:
yes
Details on sampling:
HPLC method with UV detection at 265nm
Samples taken at T=0, 2.4hrs and 5 days.
Buffers:
ph 4.00, 7.00 and 9.00
Details on test conditions:
performed at 50 C and pH 4.00, 7.00 and 9.00 with a stating conc. of c. 20mcg/ml at pH 7 (representing < 0.01M)
Duration of testopen allclose all
Duration:
2.4 h
pH:
8.95
Temp.:
50 °C
Initial conc. measured:
ca. 0.004 g/L
Duration:
5 d
pH:
8.95
Temp.:
50 °C
Initial conc. measured:
ca. 0.004 g/L
Number of replicates:
Two
Positive controls:
yes
Remarks:
Spiked soln at pH4.
Negative controls:
yes
Remarks:
Bufferer solns pH4, 7 and 9

Results and discussion

Preliminary study:
32.1% hydrolysis at 50°C after 2.4hrs
Test performance:
The pH of the test solls remained constant over the duration of the test.

Any other information on results incl. tables

Recovery of spiked solutions: 105%

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
A-87439.1 has been found to undergo significant hydrolysis at pH 4, 7 and 9.
At 25°C the test substance will possess a half-life of between 1 day and 1 year at pH4, and less than 1 day at pH 7 and 9.
Executive summary:

Hydrolysis at 2.4hrs at 50°C was 32.1% at pH 4, 91.8% at pH 7 and 100% at pH 9

Hydrolysis at 5 days at 50°C was 93.9% at pH 4 (after correction for instability)