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EC number: 420-380-5
CAS number: 136465-81-1
Control Blank -Oxygen depletions are observed throughout the 35 day
period that readings were taken. Oxygen depletions are lowest within
this system, as would be expected.
PTH-Decahydroamide -Throughout the course of the study rates of oxygen
depletion in the test system containing PTH-Decahydroamide demonstrated
very close resemblence to the pattern of oxygen depletion observed in
the Control Blank with Inoculum test system , until Day 28, when an
apparent mean degradation of 6.1 % was observed. Based on this result,
and despite the fact that PTH-Decahydroamide had already failed the test
for classification of "readily biodegradable", the study was continued
for a further 7 day period to assess the situation, utilising incubated
contingency samples. Results derived from the subsequent Day 35 samples
demonstrated little difference from the Control Blank with Inoculum
samples, and suggested a mean biodegradation of only 1.5 %. On account
of no apparent biodegradation taking place, sub-samples of incubated
samples taken for potential NO,/NO, determinations were not analysed, as
ThOD correction was not deemed necessary under these circumstances. The
test substance did not appear to exhert any inhibitory effects toward
the microbial population (see below).
PTH-Decahydroamide plus Dobanic Acid 103 -Oxygen depletion registered
after 7 days incubation represents an apparent degradation of 49.1 %,
62.6 % at Day 14, 82.9 % at Day 21, and 76.4 % at Day 28. These values
are calculated based on only the ThOD of Dobanic Acid 103, since
PTH-Decahydroamide demonstrated no biodegradation when incubated alone.
This test system does however demonstrate a slightly more rapid rate of
degradation than Dobanic Acid 103 incubated alone, indicating that
degradation of PTH-Decahydroamide may be taking place in this test
system, possibly due to a flurry of activity, and an increase in
microbial population experienced within the test system due to the
breakdown of Dobanic Acid 103.
PTH-Decahydroamide plus Sodium Acetate -This test system demonstrated a
similar pattern of oxygen depletion as the reference system containing
Sodium Acetate only (based on Sodium Acetate ThOD only), again
indicating that PTH-Decahydroamid did not act as a direct inhibitor of
microbial activity. Following a period of 7 days incubation, there was
apparently 82.2 % degradation, at 14 days this value was calculated to
be slightly less (77.0 %), at 21 days represented 105.8 %, and 28 days
105.8 %, also (all values calculated on the ThOD of Sodium Acetate only).
Dissolved oxygen determined in a sub-sample of aerated MilliQ dilution
water on Day 0 was found to contain 7. 85 mg 0,/L.
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