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Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
18 December, 1990 - 19 December, 1990
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Justification for type of information:
Non-GLP study.
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
no
Qualifier:
according to guideline
Guideline:
ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
Deviations:
no
GLP compliance:
no
Specific details on test material used for the study:
Lot No. 183831;
Purity : 97.3%.
Analytical monitoring:
no
Remarks:
Non-substance specific analytics. Oxygen electrode to follow respiration of sludge.
Vehicle:
yes
Remarks:
Test substance solutions prepared in ethanol.
Details on test solutions:
Four test item concentrations were tested, in duplicate incubates :
30 mg/L
90 mg/L
270 mg/L
484 mg/L
Duplicate Controls were also employed
Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
Activated sludge obtained from the biological waste treatment plant at Aire, City of Geneva. The sludge was prepared and used on the same day of collection.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Test temperature:
20 °C
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 100 - < 200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: Average inhibition of respiration rate at Nominal exposure concentration of 90 mg/L was 39%
Details on results:
The average inhibition of respiration observed :
30 mg/L 5.5%
90 mg/L 39.0%
270 mg/L 86.5%
484 mg/L 91.5%
Results with reference substance (positive control):
One test concentration of 3,5-dichlorophenol was tested : 40 mg/L. This concentration level demonstrated 78% inhibition of respiration.

Validity criteria fulfilled:
not specified
Conclusions:
The 3 hour EC50 of Florhydral reported in the report is 100 to 200 mg/L.

The average inhibition of respiration observed :
30 mg/L 5.5%
90 mg/L 39.0%
270 mg/L 86.5%
484 mg/L 91.5%
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
13 May, 1993 - 14 May, 1993
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
no
GLP compliance:
yes
Specific details on test material used for the study:
Lot No. 213739;
Purity 97.6%.
Analytical monitoring:
no
Remarks:
Non-substance specific analytics. Dissolved oxygen was measured in a flat bottom (BOD) flask using an O2 electrode.
Vehicle:
yes
Remarks:
Test item solutions prepared in Ethanol.
Details on test solutions:
Five concentration levels were tested of Nominal concentration 10, 32, 100, 320 and 1000 mg/L.
Test organisms (species):
activated sludge of a predominantly domestic sewage
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Test temperature:
22 °C
pH:
pH in the Controls and Nominal exposure concentrations of 10, 32 and 100 mg/L were 7.0. In the 320 and 1000 mg/L exposure solutions was a pH of 7.5.
Reference substance (positive control):
yes
Remarks:
3,5 - dichlorophenol
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 50 - < 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: 3 hour EC50 reported to be between 50 and 100 mg/L, but probably closer to 100 mg/L. An inhibition of 53% was observed for the 100 mg/L incubates. No respiration inhibition effects were observed at the 32 mg/L test concentration.
Key result
Duration:
3 h
Dose descriptor:
EC0
Effect conc.:
32 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks on result:
other: The 3-hour inhibition rate observed for the 32 mg/L incubate was -6%.
Results with reference substance (positive control):
The 3 hour EC50 for 3,5-dichlorophenol should be within the range of 5 to 30 mg/L.

The reference substance was tested at both 5 mg/L and 30 mg/L Nominal. The % inhibition of respiration observed were :
3% at 5 mg/L
68 % at 30 mg/L.


Results :


Validity criteria fulfilled:
yes
Conclusions:
The 3-hour EC50 for inhibition of sewage sludge respiration is reported as between 50 and 100 mg/L. The result for the 100 mg/L incubate demonstrates an inhibition rate of 53%, suggesting that the 3-hour EC50 is closer to 100 mg/L rather than 50 mg/L.

In addition, the EC0 for the test is 32 mg/L.

Description of key information

The 3-hour EC50 for inhibition of sewage sludge respiration is reported as between 50 and 100 mg/L. The result for the 100 mg/L incubate demonstrates an inhibition rate of 53%, suggesting that the 3-hour EC50 is closer to 100 mg/L rather than 50 mg/L.


The EC0 for the test is 32 mg/L and is used as the relevant end-point for the assessment.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:
32 mg/L

Additional information

The 3-hour EC50 for inhibition of sewage sludge respiration is reported as between 50 and 100 mg/L. The result for the 100 mg/L incubate demonstrates an inhibition rate of 53%, suggesting that the 3-hour EC50 is closer to 100 mg/L rather than 50 mg/L.


The EC0 for the test is 32 mg/L and is used as the relevant end-point for the assessment.

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