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Toxicity to soil macroorganisms except arthropods

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toxicity to soil macroorganisms except arthropods: short-term
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
key study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP Guideline study but seven deviations from protocol are listed and no analytical monitoring reported.
according to guideline
OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
GLP compliance:
Analytical monitoring:
not specified
Details on preparation and application of test substrate:
Appropriate amounts of industrial sand were coated (500 mg of MDI added to 100ml acetone + 350 g of sand mixing and drying for 2 days in furne-cupboard) with the test substance and mixed with kaolin clay -sphagnum peat mixture to reach a concentration of 1000 mg MDI per kg of dry soil. Demineralised water added to soak into the artificial soil.
Artificial soil comprised finely ground sphagnum peat, kaolin clay and fine industrial sand (ratio 1:2:7 added carbonate calcium to reach final pH of 6.0 ± 0.5)
Test organisms (species):
Eisenia fetida
Animal group:
Details on test organisms:
- Source: NIAC-proefdierbedrijf, Beers (The Netherlands).
- Age/size/weight/loading: between 300-600 mg (instead of 600 ± 100 mg according to guideline).
- Feeding: not reported.
- Pretreatment: not reported.
- Feeding during test: not reported.
Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
Total exposure duration:
14 d
Post exposure observation period:
Test parameter: mortality, weight increase, behaviour and appearance.
Test temperature:
20 ± 2 °C
6.3 (at start)
6.4 (at end)
49% (at end)
Details on test conditions:
Number of replicates, individual per replicate: 10 worms in each container, five replicates for both test containers and controls.
Control: yes (with acetone)
Nominal and measured concentrations:
1000 mg per kg of dry soil
Reference substance (positive control):
not specified
Not reported.
14 d
Dose descriptor:
Effect conc.:
> 1 000 mg/kg soil dw
Basis for effect:
other: mortality, weight, behaviour, appearance
14 d
Dose descriptor:
Effect conc.:
>= 1 000 mg/kg soil dw
Basis for effect:
other: mortality, weight, behaviour, appearance

No mortality or perturbation noted on any of the parameters.

Description of key information

A 14d-EC50 of >1000 mg/kg soil (d.w.) was obtained for the test substance for the earthworm Eisenia fetida according to OECD 207.
No long-term terrestrial tests have been performed due to the hydrolytic instability of the test substance.

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:
1 000 mg/kg soil dw

Additional information

The test substance is covered by the category approach of methylenediphenyl diisocyanates (MDI). Hence, data of the category substances can be used to cover this endpoint. A short-term toxicity study (van der Hoeven et al, 1992), carried out according to OECD 207, is available for PMDI for the earthworm Eisenia fetida. In this limit test, a 14d-EC50 of >1000 mg/kg soil (d.w.) was obtained in artificial soil. The endpoint was based on mortality, weight increase, behaviour and appearance.

No long-term terrestrial tests on soil macro-organisms are available for the test substance. Because MDI reacts readily with water to form predominantly insoluble and inert polyurea (Yakabe et al, 1994), long-term terrestrial toxicity tests are not appropriate for the derivation of PNEC values.

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