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EC number: 220-006-9
CAS number: 2601-33-4
The study was conducted
in accordance with the OECD Guidelines for the Testing of Chemicals
No.201 "Freshwater Alga and Cyanobacteria, Growth Inhibition Test"
The test was performed
with correction of the concentration by the purity (19.9%) of test
substance. There was a concern that the test substance will be adsorbed
to the test vessel. Therefore, pre-conditioning was performed using the
test solution and then the exposure test was conducted.
1) Test Organisms: Pseudokirchneriella
subcapitata, Unicellular green algae
Water: The medium recommended by the Test Guideline
3) Duration: 72 hours
4) Exposure procedure: Static, open system and skaing (100 rpm)
5) Initial biomass: 5 x 103cells/mL, algae of exponential
growth phase (dried weight of algae at the exponential growth phase: 1.8
x 10^-8 mg/cell, n=26)
6) Temperature: 22°C, variation range over the exposure period is within
7) 65 to 75 yE/m2/s,
continuous illumination with white fluorescent lamp (on the surface of
Separation factor: 1.8
* : Concentration corrected by the purity of the test substance
methods: Liquid chromatography/mass spectrometry (LC/MS)
of test substance in test solutions and test cultures
The time-weighted means of measured concentrations of the test substance
in Conc. 1 to 5were 0.0531, 0.0957, 0.179, 0.315 and 0.548 mg/L,
concentration by comparison of growth rates
The results were calculated from the time-weighted mean of measured
50% growth inhibition
concentration, ErC50 (0-72h): 0.173
mg/L (95% confidence limits: 0.154—0.194 mg/L, least squares
linear regression analysis)
No observed effect concentration, NOECr (0-7211): 0.0531
3) Observation of the appearance of algae
microscopic observation at the end of exposure, in Conc. I and 2 neither
unusual cell shape of algae (contraction, expansion, damaged cell etc.)
nor agglutination was observed, andthe algae looked normal compared with
that in the control group. The cell volume expansion was partially
observed in Conc. 3, 4 and 5.
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