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EC number: 619-217-2
CAS number: 96487-85-3
In an in vitro skin irritation test using a human skin model ( EPISKIN
Standard Model), the influence of the test substance on the viability of
human skin was tested. The test substance was applied directly to 0.38 cm2
cultured skin (at least 10 mg, in presence of 5 μl Milli-Q water). After
15 minutes, the substance was removed and cells were cultured for 42
hours. The viability of the cells was tested by reduction of MTT.
Survival of unexposed skin was set at 100%, the positive control had a
mean cell viability of 10% whereas the test substance showed cell
viability of 94%. Since the mean relative tissue viability after
exposure to the test substance was above 50%, it can be concluded that
the test substance is not irritating in the in vitro skin irritation
test and should not be classified according to the Globally Harmonized
System of Classification and Labeling of Chemicals (GHS) of the United
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