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EC number: 500-147-5
CAS number: 61788-85-0
1 - 6.5 moles ethoxylated
The substance was tested in the EPISKIN™ Reconstructed Human Epidermis
Model using triplicate tissues during 15 minutes. The relative mean
viability of the test substance treated tissues was 100.7 %. Therefore
it can be concluded that the substance is not irritant to the skin.
The substance was tested in the EpiDerm™ Human Skin Model using
duplicate tissues during 3 and 60 minutes. The relative mean viability
of the test substance treated tissues was 98.0 % for the 3 minutes
exposure and 101.5% for the 60 minutes exposure. Therefore it can be
concluded that the substance is not corrosive to the skin.
The eye irritation potential of the substance was assessed by
means of the Human Cornea Model Test with exposure to 50 µL of the test
item, the negative control (deionised water) or the positive control.
Irritating effects were not observed following incubation with the
substance. Compared with the value of the negative control the relative
mean absorption value corresponding to the viability of the tissues did
not decrease below 60% (92.1%).
Relative mean viability
Ab-sorbanceWell 1(Tissue 1/2)
Ab-sorbanceWell 2 (Tissue 1/2)
Mean Absor-bance (Tissue 1/2)
Mean Absorbance* Tissue 1 and 2
Mean Absorbance of2 Tissues*
Rel. Absorbance [%]Tissue 1 and 2**
Absolute Value of the Difference of the Rel. Absorbances [%]Tissue 1 and 2
Mean Rel. Absorbance
of two replicate wells after blank correction
absorbance [rounded values]: 100 * (absorbace test item/positive
control/negative control)/absorbance negative control
relative absorbance [rounded values]: 100
* (mean absorbace test item/positive control/negative control)/mean
absorbance negative control
eye irritation potential of the substance was assessed by means of the
Human Cornea Model Test. The
substance did not prove to be an MTT reducer and color interference was
50 µL of the test item, the negative control (deionised water) or the
positive control (methyl acetate) were applied to each of duplicate
tissue for 30 minutes.
treatment with the negative control the absorbance values were well
within the required acceptability criterion of OD > 0.8 and < 2.5 thus
showing the quality of the tissues.
with the positive control induced a decrease below 50% compared with the
negative control value in the relative absorbance thus ensuring the
validity of the test system.
difference of viability between the two relating tissues was < 20% in
the same run (for test item tissues, positive and negative control
effects were not observed following incubation with the substance.
Compared with the value of the negative control the relative mean
absorption value corresponding to the viability of the tissues did not
decrease below 60% (92.1%).
conclusion, it can be stated that in this study and under the
experimental conditions reported, the substance does not possess
any eye irritating potential.
In Vitro Irritancy Score
Post-Incubation - Pre‑Treatment
OD= Optical density
In a BCOP assay bovine corneas
were exposed to the substance and assessed for opacity and permeability.
No effects of the substance on opacity readings and permeability was
observed. The calculation of the In Vitro Irritancy Score (1.3) showed
that the substance is not severely damaging to the eyes.
Based on the available information no classification for skin- and eye
irritation is necessary according to Regulation (EC) No 1272/2008 (CLP).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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