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EC number: 234-282-3
CAS number: 11065-74-0
72h EC50 649.3 mg/L, the 72h EC10 and NOEC were 42.8 and 15 mg/L, respectively for Pseudokirhnerella subcapitata (OECD TG 201)
MnNa2 -EDTA was tested with multiple
media compositions, due to the apparent high toxicity of this metal EDTA
complex. A series of preliminary tests was conducted to determine the
influence of enriched medium nutrients on the initial ErC10value.
The nature of a chelating agent can cause potential nutrient depletion
in the test media. This causes a growth related effect that is not due
to substance toxicity. This series of preliminary studies was conducted
to investigate this effect.
The final test (Geurts, 2009) was
conducted with the following adjustments:
The NaHCO3 concentration of the test
medium was 150 mg/L instead of 50 mg/L, in order to help maintain a more
constant pH during the test.
The Fe-EDTA and Trace elements
components of the OECD media were increased to 150% of the standard
composition, in order to prevent
nutrient deprivation during the test.
The key study, conducted according to
OECD guideline 201 under GLP, revealed a 72h EC50 of 649.3 mg/l and a
72h NOEC of 15 mg/l for Pseudokirchnerella subcapitata. The 72h-EC10 was
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