Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 208-589-8
CAS number: 534-15-6
Individual and Mean Corneal Opacity and Permeability Measurements
Post-Incubation - Pre-Treatment
OD = Optical density
* = Mean of the post-incubation − pre-treatment values
♦ = Mean permeability
• = Mean corrected value
The purpose of this test was to identify test items that can induce
serious eye damage and to identify test items not requiring
classification for eye irritation or serious eye damage. The Bovine
Corneal Opacity and Permeability (BCOP) test method is an organotypic
model that provides short-term maintenance of normal physiological and
biochemical function of the bovine cornea in vitro. In this test method,
damage by the test item is assessed by quantitative measurements of
changes in corneal opacity and permeability.
The test method can correctly identify test items (both chemicals and
mixtures) inducing serious eye damage as well as those not requiring
classification for eye irritation or serious eye damage, as defined by
the United Nations (UN) Globally Harmonized System of Classification and
Labelling of Items (GHS) and EU Classification, Labelling and Packaging
(CLP) of chemicals (Regulation (EC) No 1272/2008), and it was therefore
endorsed as scientifically valid for both purposes. Test items inducing
serious eye damage are classified as UN GHS and EU CLP Category 1. Items
not classified for eye irritation or serious eye damage are defined as
those that do not meet the requirements for classification as UN GHS/EU
CLP Category 1 or 2 (2A or 2B), i.e. they are referred to as UN GHS/EU
CLP No Category.
The undiluted test item was applied for 10 minutes followed by an
incubation period of 120 minutes. Negative and positive control items
were tested concurrently. The two endpoints, decreased light
transmission through the cornea (opacity) and increased passage of
sodium fluorescein dye through the cornea (permeability) were combined
in an empirically derived formula to generate an In Vitro Irritancy
The test item is classified according to the prediction model as follows:
The In Vitro irritancy scores are summarized as follows:
In Vitro Irritancy Score
No prediction of eye irritation can be made.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again