Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 947-432-4
CAS number: -
1Corrected Mean Control Opacity Score = 4 hour mean score minus pretest
* = The permeability scores of the control corneas, C3 and C4, used for
the BCQP testing on 23 Jun 2014, significantly exceeded
the expected OD of untreated and intact corneas. Additionally, the C3
and C4 permeability scores exceeded those of the test
article corneas. The BCOP was repeated on 26 Jun 2014. The conclusion
was based on the BCOP performed on 26 Jun 2014.
RESULTS for 23 Jun 2014
INDIVIDUAL CONTROL SCORES FOR BCOP
RESULTS for 26 Jun 2014
INDIVIDUAL TEST SCORES
2Corrected Mean Opacity Score = mean treated opacity score minus
corrected mean control opacity score
Objective: To determine the potential for ocular irritation using an
alternative to the Draize methodology.
This protocol is based on the methodology described in "Bovine Corneal
Opacity and Permeability Test:
An In Vitro Assay of Ocular Irritancy, (1992)"; Gautheron, Pierre;
Dukic. Martine; Alix, Danielle and Sina.
Joseph F.; Fundamental and Applied Toxicology 18, 442-449. In Vitro
classification based on Southee
JA, 1998. Evaluation of the Prevalidation Process, Part 2, final report,
Volume 2, The Bovine Corneal
Opacity and Permeability (BCOP) Assay. European Community Contract No.
11279-95-10F lED ISP GB
and included an analysis based on OECD Guideline for the Testing of
Chemicals #437, adopted
September 7, 2009
Method Synopsis: Ten corneas were dosed with 0.75 ml of a 20% suspension
L-005457795-001G001. Five corneas were dosed on 23 Ju12014. Opacity
measurements and sodium
fluorescein permeability were determined. The permeability scores of the
control corneas, C3 and C4,
used for the BCOP testing on 23 Jun 2014. significantly exceeded the
of untreated and intact corneas. Additionally, the C3 and C4
permeability scores exceeded those of the test article
corneas. As a result, the BCOP was repeated on 26 Jun 2014. The
conclusion was based on the BCOP
performed on 26 Jun 2014.
Summary: The corrected mean opacity score was -1.4 The corrected mean
(permeability) score was 0.012.
Conclusion: The in vitro score was calculated as -1.22 and is classified
as a non-irritant (Southee,
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again