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EC number: 200-460-4
CAS number: 60-18-4
of the Test Item L-Tyrosine
mean OD570 of the duplicates (blank-corrected)
total mean OD570 of 3 replicate tissues (blank-corrected)
relative tissue viability [%]
mean relative tissue viability [%]
SD tissue viability [%]***
CV [% viabilities]
Blank-corrected mean OD570 nmof the negative control
corresponds to 100% absolute tissue viability.
relative tissue viability of the three positive control tissues is ≤ 20%.
deviation (SD) obtained from the three concurrently tested tissues
The potential of the test item to
induce skin irritation was analysed by using the three-dimensional human epidermis
model EpiDerm (MatTek) comprising a reconstructed epidermis with a
functional stratum corneum. The
test was performed according to OECD TG 439 and in compliance to GLP.
In the present study L-Tyrosine was
applied topically to the EpiDerm tissue for 60 min followed by a 42 h
post-incubation period and immediate determination of cytotoxic effects
via MTT reduction assay.
Irritant potential of the test item
was predicted from the relative mean tissue viabilities obtained
compared to the corresponding negative control tissues concurrently
treated with DPBS.
The test item showed no irritant
effects. The mean relative tissue viability (% negative control) was
> 50% (94.9%) after 60 min treatment and 42 h post-incubation.
The controls confirmed the validity of
the study. The mean absolute OD570of the three negative
control tissues was > 0.8 and ≤ 2.8. The mean relative tissue viability
(% negative control) of the positive control was ≤ 20%
(3.9%). Standard deviation of viability of replicate tissues of all dose
groups was ≤ 18% (0.4% - 6.0%).
In conclusion, in
this study under the given conditions the test item showed no irritant
The test item is therefore considered as
non-irritant to skin.
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