Reaction mass of Benzenamine, N,N-dimethyl- , molybdate, tungstate & phosphates

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

Data is for read across substance

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: As mention in below principle

Principles of method if other than guideline:

WoE report was prepared based on two short term toxicity study of algae for the test chemical :

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material : Reaction mass of Benzenamine, N,N-dimethyl- , molybdate, tungstate & phosphates- Substance type: Organic- Physical state: Solid- Physical Appearance: Violet powder

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

other: green algae

Test type:

static

Water media type:

freshwater

Total exposure duration:

72 h

Reference substance (positive control):

not specified

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

0.34 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 1 study

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

0.093 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: AUG

Remarks on result:

other: 1st study

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

0.15 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: 1st study

Key result

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

0.11 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: areas under the growth curves

Remarks on result:

other: 2nd study

Validity criteria fulfilled:

not specified

Conclusions:

1. Based on effect on growth rate of the test organism green algae, the 72 hr EC50 and NOEC value was determined to be 0.34 and 0.026 mg/l, respectively and on the basis of AUG, the 72 hr EC50 and NOEC value was determined to be 0.093 and 0.026 mg/l, respectively. 2. After the exposure of 72 hrs of test chemical with green algae effect on the growth rate and on area under the growth curve were observed. The EC50 and NOEC on the basis of growth rate was determine to be 0.15 and 0.027 mg/l. And on the basis of area under the growth curve the EC50 and NOEC was determine to be at 0.11 and 0.037 mg/l.Based on above experimental studies it was concluded that the Reaction mass of Benzenamine, N,N-dimethyl- , molybdate, tungstate & phosphates was toxic to the growth of algae and classified as aquatic acute 1 and chronic 1 as per the CLP criteria.

Executive summary:

Summarized results of the data available for the structurally and functional similar read across chemicals study has been reviewed to determine the toxicity of the test reaction mass of Benzenamine, N,N-dimethyl- , molybdate, tungstate & phosphates on the growth of aquatic algae. The studies are as mentioned below:

 

In the first study short term toxicity to green algae study was carried out for 72 hrs. The study was based on the effects of the test compound on green algae in a static fresh water system. Based on effect on growth rate of the test organism green algae, the 72 hr EC50 and NOEC value was determined to be 0.34 and 0.026 mg/l, respectively and on the basis of AUG, the 72 hr EC50 and NOEC value was determined to be 0.093 and 0.026 mg/l, respectively. Thus, based on the EC50 value, it can be concluded that the substance can be considered as toxic to aquatic organisms and thus can be considered to be classified in aquatic acute category 1 / chronic 1 as per the CLP classification criteria.

 

Similarly aim of the second study was to evaluate the nature of chemical when comes in contact with green algae by providing the exposure period of 72 hrs. Test conducted under the static system. After the exposure of 72 hrs of test chemical with green algae effect on the growth rate and on area under the growth curve were observed. The EC50 and NOEC on the basis of growth rate was determine to be 0.15 and 0.027 mg/l. And on the basis of area under the growth curve the EC50 and NOEC was determine to be at 0.11 and 0.037 mg/l.

 

Based on above experimental studies it was concluded that the reaction mass of Benzenamine, N,N-dimethyl- , molybdate, tungstate & phosphates was toxic to the growth of algae and classified as aquatic acute 1 and chronic 1 as per the CLP criteria.

Description of key information

1. Based on effect on growth rate of the test organism green algae, the 72 hr EC50 and NOEC value was determined to be 0.34 and 0.026 mg/l, respectively and on the basis of AUG, the 72 hr EC50 and NOEC value was determined to be 0.093 and 0.026 mg/l, respectively.

2. After the exposure of 72 hrs of test chemical with green algae effect on the growth rate and on area under the growth curve were observed. The EC50 and NOEC on the basis of growth rate was determine to be 0.15 and 0.027 mg/l. And on the basis of area under the growth curve the EC50 and NOEC was determine to be at 0.11 and 0.037 mg/l.

Based on above experimental studies it was concluded that the reaction mass of Benzenamine, N,N-dimethyl- , molybdate, tungstate & phosphates was toxic to the growth of algae and classified as aquatic acute 1 and chronic 1 as per the CLP criteria.

Key value for chemical safety assessment

EC50 for freshwater algae:

0.34 mg/L

Additional information

Summarized results of the data available for the structurally and functional similar read across chemicals study has been reviewed to determine the toxicity of the test reaction mass of Benzenamine, N,N-dimethyl- , molybdate, tungstate & phosphates on the growth of aquatic algae. The studies are as mentioned below:

 

In the first study short term toxicity to green algae study was carried out for 72 hrs. The study was based on the effects of the test compound on green algae in a static fresh water system. Based on effect on growth rate of the test organism green algae, the 72 hr EC50 and NOEC value was determined to be 0.34 and 0.026 mg/l, respectively and on the basis of AUG, the 72 hr EC50 and NOEC value was determined to be 0.093 and 0.026 mg/l, respectively. Thus, based on the EC50 value, it can be concluded that the substance can be considered as toxic to aquatic organisms and thus can be considered to be classified in aquatic acute category 1 / chronic 1 as per the CLP classification criteria.

 

Similarly aim of the second study was to evaluate the nature of chemical when comes in contact with green algae by providing the exposure period of 72 hrs. Test conducted under the static system. After the exposure of 72 hrs of test chemical with green algae effect on the growth rate and on area under the growth curve were observed. The EC50 and NOEC on the basis of growth rate was determine to be 0.15 and 0.027 mg/l. And on the basis of area under the growth curve the EC50 and NOEC was determine to be at 0.11 and 0.037 mg/l.

 

Based on above experimental studies it was concluded that the reaction mass of Benzenamine, N,N-dimethyl- , molybdate, tungstate & phosphates was toxic to the growth of algae and classified as aquatic acute 1 and chronic 1 as per the CLP criteria.