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EC number: 215-148-3
CAS number: 1306-24-7
Number and percentage of immobilised animals
[% sat. sol.]
: statistically significantly different compared to the control values
Acute toxicity of Cadmium selenide on Daphnia magna was assessed in an
acute immobilisation test, over an exposure period of 48 hours in a
semi-static test system. Even if significant immobility was not observed
during the preliminary range-finding test, however, comparing the
preliminary range-finding test with the main test (which was stopped)
large differences can be observed at the solubility level of the test
item (100% saturated solution). Therefore five test concentrations in a
geometric series (using factor 3.2) and one control were tested in the
definitive test under semi-static conditions.
The nominal concentrations of test item used in the main experiment
were: 1.0, 3.1, 9.8, 31.3 and 100.0 % saturated solutions.
The test concentrations were analytically determined at the start and at
the end of the renewal periods by measuring the concentration of Cadmium
(as a marker for the test item) using a suitable ICP method. The
corresponding measured geometric mean test item concentrations were:
11.38, 34.36, 107.88, 344.78 and 1146.99 μg/L (based on the calculated
Cadmium content of 58.737 % in the test item).
The biological results are based on both the nominal percentage
saturated solutions and measured geometric mean test item concentrations.
Twenty animals, divided into four groups (glass beaker) of five animals
each were used at the test concentrations and for the control as well.
The 24 and 48 hours EC50 values of the test item and their confidence
limits were calculated using Probit analysis by TOXSTAT software. The
48h EC100 values of the test item were determined directly from the raw
For the determination of the LOEC and NOEC, the immobilization at the
test concentrations was tested on significant differences to the control
values by Dunnett’s Test using TOXSTAT software.
All validity criteria were met during this study.
Under the conditions of this Daphnia magna acute immobilisation study
the observed endpoints for the effect of Cadmium selenide were the
Based on the nominal concentration (% saturated solution of 100 mg/L
nominal loading rate):
The 24h EC50 value: 21.76% saturated solution (nominal) (95 % conf.
limits: 18.72 – 25.28% saturated solution)
The 48h EC50 value: 2.80% saturated solution (nominal) (95 % conf.
limits: 2.30 – 3.40% saturated solution)
The 48h EC100 value: 100.0% saturated solution (nominal)
The 48h No-Observed Effect Concentration (NOEC): 1.0% saturated solution
The 48h Lowest Observed Effect Concentration (LOEC): 3.1% saturated
Based on the measured test item concentrations:
The 24h EC50 value: 241.06 μg/L (measured) (95 % conf. limits: 207.18 –
The 48h EC50 value: 31.20 μg/L (measured) (95 % conf. limits: 25.71 –
The 48h EC100 value: 1146.99 μg/L (measured)
The 48h No-Observed Effect Concentration (NOEC): 11.38 μg/L (measured)
The 48h Lowest Observed Effect Concentration (LOEC): 34.36 μg/L
Acute toxicity of Cadmium selenide was assessed with Acute
immobilisation test on Daphnia magna, over an exposure period of 48
hours in a semi-static system.
Based on the measured test item concentrations: The 24h EC50 value:
241.06 μg/L (measured) (95 % conf. limits: 207.18 – 280.49 μg/L)
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