Registration Dossier
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 216-407-3 | CAS number: 1576-35-8
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2004
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study was conducted in accorance to OECD Guideline No. 211 and in compliance with GLP
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
no data - Analytical monitoring:
- yes
- Vehicle:
- yes
- Details on test solutions:
- Stock solution was prepared by diluting test chemical in dimethylformamide (200,000 mg/L). Test solutions were prepared by adding the corresponding amounts of stock solution into 1,000 mL of OECD medium. The concentration of solvent was 100 µL/L.
OECD M4 medium was applied.
Test concentrations were measured 3 times during 21 days before and after renewal of test solutions - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Age: Juveniles within 24 hrs old
Supplier: Independent Administrative Institution National Institute of Environmental Studies, Japan
Daphnids were fed Chlorella vulgaris for 0.15 mg C /day/animal during the test periods. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Post exposure observation period:
- no
- Hardness:
- 250 mg/L as CaCO3
- Test temperature:
- 20 ± 1 °C
- pH:
- 7.5 ~ 8.8
- Dissolved oxygen:
- 7.3 ~ 8.6 mg/L (over 60 % of the air saturation value)
- Nominal and measured concentrations:
- Nominal
Con.
(mg/L) Measured Con. (mg/L) TWM** (mg/L) % of Nominal
0 day (new) 1 day (old) 7 day (new) 8 day (old) 14 day (new) 15 day (old)
mg/L (%*) mg/L (%*) mg/L (%*) mg/L (%*) mg/L (%*)
Control <0.002 (–) <0.002 (–) <0.002 (–) <0.002 (–) <0.002 (–) <0.002 (–) – –
Solvent Control <0.002 (–) <0.002 (–) <0.002 (–) <0.002 (–) <0.002 (–) <0.002 (–) – –
0.400 0.370 (93) 0.155 (39) 0.335 (84) 0.143 (36) 0.327 (82) 0.129 (32) 0.229 57
0.850 0.779 (92) 0.332 (39) 0.706 (83) 0.298 (35) 0.760 (89) 0.288 (34) 0.495 58
1.80 1.59 (88) 0.684 (38) 1.68 (93) 0.633 (35) 1.60 (89) 0.674 (37) 1.07 59
3.80 3.32 (87) 1.33 (35) 3.28 (86) 1.32 (35) 3.09 (81) 1.29 (34) 2.13 56
8.00 7.05 (88) 2.74 (34) 6.94 (87) 2.69 (34) 6.53 (82) 2.72 (34) 4.47 56 - Details on test conditions:
- Light: 800 Lux, Light periodicity: 16/8 (light/dark)
- Reference substance (positive control):
- not specified
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 2.48 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LC50
- Effect conc.:
- 2.13 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 2.13 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 4.47 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Details on results:
- 21day-EC50 (95 % confidence limits) of OBSH to Daphnia magna was 2.48 mg/L (2.31 ~ 3.63 mg/L). At the end of the test, the mortality of the parent daphnids was 0 % and 10 % in the control and solvent control, respectively. The mean number of live offspring produced per parent animal surviving at the end of the test was 92 animals and 107 animals in control and solvent control, respectively.
- Results with reference substance (positive control):
- no data
- Reported statistics and error estimates:
- The LC50 value and the 95 % confidence limits were calculated by Probit Analysis or Binominal or Moving average method. The EC50 value and the 95 % confidence limits were calculated by Logistic regression analysis method. The NOEC and the LOEC value were analysed by Bartlett test and 1-way ANOVA or Kruskal-Wallis test.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- EC50 (21 d) = 2.48 mg/L
NOEC = 2.13 mg/L - Executive summary:
The chronich toxicity of OBSH towards Daphnia magna was assessed applying the OECD Guidline No. 211.
The following concentrations were tested: Control, Solvent control,0.400, 0.850, 1.80, 3.80 and 8.00 mg/L
Based on the results a 21day-EC50(95 % confidence limits) of OBSH to Daphnia magna was determined to be 2.48 mg/L (2.31~3.63 mg/L) the 21day NOEC was 2.13 mg/L. At the end of the test, the mortality of the parent daphnids was 0 % and 10 % in the control and solvent control, respectively. The mean number of live offspring produced per parent animal surviving at the end of the test was 92 animals and 107 animals in control and solvent control, respectively.
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Read-across from long-term toxicity study in daphnia on one closely structural and physicochemical related substance: OBSH (4,4'-Oxybis (benzenesulfonyl hydrazide)).
For further read-across justification se document attached in section 13. - Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 2.13 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction
- Conclusions:
- Based on read-across to OBSH (80-51-3), the 21 -d NOEC(daphnia, 48hrs) is derived at 2.13 mg/L
- Executive summary:
Based on read-across to OBSH (80-51-3), the 21 -d NOEC(daphnia, 48hrs) is derived at 2.13 mg/L
Referenceopen allclose all
21day-EC50(95 % confidence limits) of OBSH to Daphnia magna was 2.48 mg/L (2.31~3.63mg/L). At the end of the test, the mortality of the parent daphnids was 0 % and 10 % in the control and solvent control, respectively. The mean number of live offspring produced per parent animal surviving at the end of the test was 92 animals and 107 animals in control and solvent control, respectively.
Description of key information
Based on read-across to OBSH (80-51-3), the 21 -d NOEC(daphnia, 48hrs) is derived at 2.13 mg/L. A 21 -d reproduction study on Dapnhia magna for OBSH showed an NOEC of 2.13 mg/L (GOECD 211). Therefore, the 21-d NOEC(Daphnia) for target substance is derived at 2.13 mg/L
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 2.13 mg/L
Additional information
Based on read-across to OBSH (80-51-3), the 21 -d NOEC(daphnia, 48hrs) is derived at 2.13 mg/L. A 21 -d reproduction study on Dapnhia magna for OBSH showed an NOEC of 2.13 mg/L (GOECD 211). Therefore, the 21-d NOEC(Daphnia) for target substance is derived at 2.13 mg/L
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Route: .live2