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EC number: 211-189-6
CAS number: 632-99-5
Adsorption / desorption
The adsorption coefficient Koc in soil
and in sewage sludge of test chemical
hydrochloride (CAS No. 632 -99 -5) was determined by the Reverse Phase
High Performance Liquid Chromatographic method according to OECD
Guideline No. 121 for testing of Chemicals (Experimental study report,
2018). The solutions of the test substance and reference substances were
prepared in appropriate solvents. A test item solution was prepared by
accurately weighing 4 mg of test item and diluted with Methanol up to 10
ml. Thus, the test solution concentration was 400 mg/l. The pH of test
substance was 6.3. Each of the reference substance and test substance
were analysed by HPLC at 210 nm. After equilibration of the HPLC system,
Urea was injected first, the reference substances were injected in
duplicate, followed by the test chemical solution in duplicate.
Reference substances were injected again after test sample, no change in
retention time of reference substances was observed. Retention time tR
were measured, averaged and the decimal logarithms of the capacity
factors k were calculated. The graph was plotted between log Koc versus
log k(Annex - 2).The linear regression parameter of the relationship log
Koc vs log k were also calculated from the data obtained with
calibration samples and therewith, log Koc of the test substance was
determined from its measured capacity factor. The reference substances
were chosen according to functional similarity with the test substance
and calibration graph prepared. The reference substances were Benzamide,
N-methylbenzamide, 4-Nitrobenzamide, N,N-dimethylbenzamide, 4
-methylaniline (p-Tolouidine), 4 -chloroaniline, Nitrobenzene, 2 -
nitrophenol having Koc value ranging from 1.239 to 2.47. The Log Koc
value of test chemical (4 -(4 -aminophenyl)(4 -iminocyclohexa-2,5
-dienylidene)methyl)-2 -methylaniline hydrochloride was determined to be
1.908 ± 0.009 at 25°C. This log Koc value indicates that the substance
(4 -(4 -aminophenyl)(4 -iminocyclohexa-2,5 -dienylidene)methyl)-2
-methylaniline hydrochloride has a low sorption to soil and sediment and
therefore have moderate migration potential to ground water.
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