Trisodium [5-acetamido-4-hydroxy-3-[[2-hydroxy-4-[[2-(sulphooxy)ethyl]sulphonyl]phenyl]azo]naphthalene-2,7-disulphonato(5-)]cuprate(3-)

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

09 - 18 Jan 1991

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Reference 2

Reference Type:

other company data

Title:

Unnamed

Year:

1993

Report date:

1993

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Trisodium [5-acetamido-4-hydroxy-3-[[2-hydroxy-4-[[2-(sulphooxy)ethyl]sulphonyl]phenyl]azo]naphthalene-2,7-disulphonato(5-)]cuprate(3-)

EC Number:

263-856-6

EC Name:

Trisodium [5-acetamido-4-hydroxy-3-[[2-hydroxy-4-[[2-(sulphooxy)ethyl]sulphonyl]phenyl]azo]naphthalene-2,7-disulphonato(5-)]cuprate(3-)

Cas Number:

63105-49-7

Molecular formula:

C20H14CuN3O15S4.3Na C20H14CuN3Na3O15S4

IUPAC Name:

trisodium [5-acetamido-4-hydroxy-3-[[2-hydroxy-4-[[2-(sulphooxy)ethyl]sulphonyl]phenyl]azo]naphthalene-2,7-disulphonato(5-)]cuprate(3-)

Test material form:

solid: particulate/powder

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

At 48 and 96 h removal of ca. 100 mL test solution from the middle of the aquarium (T =22°C)

Test solutions

Vehicle:

no

Details on test solutions:

The test item was dissolved in test medium, homogenised with Ultra-Turrax and in ultrasonic bat, then directly mixed into test medium in aquarium

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebrafish
- Source: West Aquarium, Bad Lauterberg, Germany
- Age at study initiation: 3-4 months
- Length at study initiation (length definition, mean, range and SD): 2.6 - 3.3 cm, x=2.9 cm, s=0.27 cm (n=10)

ACCLIMATION
- Acclimation period: min. 14 d
- Type and amount of food during acclimation: ad lib.
- Feeding frequency during acclimation: daily

FEEDING DURING TEST
None

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Test temperature:

21.2 - 22.9°C

pH:

7.8 - 8.3

Dissolved oxygen:

7.7 - 9.6 mg/L

Nominal and measured concentrations:

Nominal: 0 (control), 500 mg/L
Arithmetic mean measured: 499.7 mg/L (99.9% recovery)

Details on test conditions:

TEST SYSTEM
- Test vessel: aquarium
- Material, size, headspace, fill volume: glass, 30 x 22 x 24 cm, 10 L fill volume
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
Reconstituted water accroding to ISO/DIS 7346/1

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 12/12

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Mortality and appearance/behaviour at 2-4, 24, 48, 72 and 96 h after start of exposure

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 500 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

0% mortality in negative control and 500 mg/L treatment
Behaviour and appearance observations limited due to strong dark violet discolouration of test medium, behavioural abnormalities observed

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The test item did not cause any acutely toxic effect to fish (Danio rerio). The 96-h LC50 was >500 mg/L (analytically verified). The test item is the hydrolyzed form of the reactive dye. Since reactive dyes moderately hydrolyse in water at neutral pH conditions, this is considered the environmentally relevant form of the test item.