Tungsten disulphide

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2001-12-11, 2002-02-01

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
-Source and lot.batch No. of test material: 16031/01
-Purity test date: >98%

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

Stock Solution: The solution (600 mg/L test item were weighed out) was applied to 6 L test medium (8 parts with 75 mg/ 750 mL)
Dispersion Treatment: The stock solution was shaken with 20 upm for 24 h on a magnetic stirrer and filtrated with 0.45 um
Limit Concentration: Saturated Solution (saturated concentration)

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Danio rerio (zebrafish)
-Origin: Aquarium AM Aegi, FeldstraBe 7, D-30159 Hannover

ACCLIMATION
-Acclimation period: 12days
-Acclimation conditions (same as test or not): 22 degree celcius and diffuse light, water was changed once per week
-Type and amount of food: Trouvit 40/2; Milkivit, D-86666 Burgheim. 4% of the fish body weight feeding per day
- Feeding Frequency: 3 times per week

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

Number of Zebrafish incapable of swimming after 24, 48, 72, and 96 h

Hardness:

Total hardness of 40 to 180 mg CaC)3/L

Test temperature:

22 +\- 2 degree C

pH:

6.0-8.5

Dissolved oxygen:

not less than 80% of air saturation value

Nominal and measured concentrations:

The mean measured concentration was 360 ug/L for the test item Tungsten Disulphide

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass-aquaria loosely covered by glass tops
- Material, size, headspace, fill volume: 5 L per vessel
- No. of organisms per vessel: 7 zebrafish for concentration and cotrol
- No. of vesseles per concentration (replicates): one per concentration and control

RANGE-FINDING STUDY?

Reference substance (positive control):

yes

Remarks:

7 zebrafish in dilution water (without test item)

Key result

Duration:

2 h

Dose descriptor:

LC50

Effect conc.:

> 360 µg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

> 360 µg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 360 µg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

> 360 µg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 360 µg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

yes

Conclusions:

The test concentration of 360 ug/L (mean measured concentration) caused no mortality or non lethal effects. Therefore the NOEC was laid down as 360 ug/L (mean measured concentration)

Executive summary:

There is short-term toxicity to fish data available for tungsten disulphide.The acute toxicity of the test item tungsten disulphide to fish (Zebrafish) was determined according to EEC Directive 92/69/C1. A saturated solution was tested in a static limit test as single test concentration selected on the basis of a preliminary range finding test. Duration of the test was 96 h. 7 test organisms were exposed to the test concentration and the control and recorded at every 2, 24, 48, 72, and 96 hour. Concentration control analysis was carried out and reported by an external GLP laboratory. Tungsten contents were found to be stable during the test period. The mean measure concentration was 360 ug tungsten/L

The LC-Values with 95% confidence intervals are: EC50 at 2 h: >360 ug/L, EC50 at 24h: >360 ug/L, EC50 at 48h: >360 ug/L, EC50 at 72h: >360 ug/L, and EC50 at 96h: >360 ug/L

On the basis of observations made during the test, the NOEC was determined at 360 ug/L for the test item tungsten disulphide. In this study tungsten disulphide was not found to be toxic to zebrafish after 96h at the mean measured concentration of 360 ug/L

Description of key information

There is short-term toxicity to fish data available for tungsten disulphide. The acute toxicity of the test item tungsten disulphide to fish (Zebrafish) was determined according to EEC Directive 92/69/C1. A saturated solution was tested in a static limit test as single test concentration selected on the basis of a preliminary range finding test. Duration of the test was 96 h. 7 test organisms were exposed to the test concentration and the control and recorded at every 2, 24, 48, 72, and 96 hour. Concentration control analysis was carried out and reported by an external GLP laboratory. Tungsten contents were found to be stable during the test period. The mean measure concentration was 360 ug tungsten/L

The LC-Values with 95% confidence intervals are: EC50 at 2 h: >360 ug/L, EC50 at 24h: >360 ug/L, EC50 at 48h: >360 ug/L, EC50 at 72h: >360 ug/L, and EC50 at 96h: >360 ug/L

On the basis of observations made during the test, the NOEC was determined at 360 ug/L for the test item tungsten disulphide. In this study tungsten disulphide was not found to be toxic to zebrafish after 96h at the mean measured concentration of 360 ug/L

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

360 µg/L