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EC number: 813-789-9
CAS number: 152873-67-1
Fluormorpholinylacrylaldehyd (FMA): Acute Toxicity to
Daphnia magna in a Static 48-hour Immobilisation Test
The purpose of this study was to determine the influence
of the test item Fluormorpholinylacrylaldehyd (FMA) on the mobility of
For this purpose, young daphnids (< 24 hours old) were
exposed in a static test to various concentrations under defined
conditions for 48 hours. The recorded effects were the mobility of the
daphnids after 24 and 48 hours. The test method of application and the
test system are recommended by the test guidelines and Daphnia magna is
the recommended test species. The purpose of the analytical part of this
study was to verify the concentrations of the test item in the test
-OECD Guideline for Testing of Chemicals No. 202:
"Daphnia sp., Acute Immobilisation Test" adopted April 13, 2004
-SANCO/3029/99 rev.4 11/07/00: Residues: Guidance for
generating and reporting methods of analysis in support of
pre-registration data requirements for Annex II (part A; Section 4) and
Annex III (part A; Section 5) of directive 91/414
Materials and Methods
Fluormorpholinylacrylaldehyd (FMA); batch no.: BXR82PT;
purity: 98.6 %, according to certificate of analysis
Female Daphnia magna, clone 5; 1.5 to 20 hours old
Source: The daphnids introduced in the test were taken
from ibacon's in-house laboratory culture.
This study encompassed 6 treatment groups (5 dose rates
of the test item and a control) each containing 20 individuals. The
mobility of the daphnids was determined in a static 48-hour test by
visual observation after 24 and 48 hours.
Number of immobile organisms after 24 and 48 hours
10, 4.5, 2.1, 0.9 and 0.4 mg test item/L (spacing factor
2.2) and a control.
Water temperature: 19.7 to 20.3 °C; pH value: 7.5 to 7.9;
dissolved oxygen concentration: 8.8 to 8.9 mg/L; photoperiod: 16 h light
- 8 h dark; light intensity: 210 to 370 lux; and thus were within the
ranges requested by guideline OECD 202
Biological test results:
After 48 hours of exposure no immobilisation of the test
animals was observed in the control and up to the test item
concentration of 2.10 mg test item/L. At the concentration of 4.5 mg
test item/L 10 daphnids were immobile. At the highest test item
concentrations of 10.0 mg test item/L 20 daphnids were immobile,
Analytical test results:
The quantification of the test item
Fluormorpholinylacrylaldehyd (FMA) in the test samples was performed
using liquid chromatography with UV detection.
At the start of the test and after 48 hours test duration
102 % of the nominal test concentrations were found (average of all test
The toxic effect of the test item
Fluormorpholinylacrylaldehyd (FMA) to Daphnia magna was assessed in a
static concentration-response test. The 48-hour NOEC was determined to
be 2.1 mg test item/L. The 48-hour LOEC was determined to be 4.5 mg test
item/L and the 48-hour EC50 value was determined to be 4.5 mg test
The initial concentrations and the maintenance of the
exposure concentrations during the test were verified in the analytical
part. All reported results refer to nominal values since the
concentrations of the test item were within ± 20% of the nominal
concentrations during the test. This
study is classified as acceptable and satisfies the guideline
requirements for an acute toxicity study with freshwater invertebrates.
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