Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.

REACH

Tripiperazine dicitrate

EC number: 205-622-8 | CAS number: 144-29-6

Life Cycle description
Uses advised against

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed using standard test guidelines

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

Specific details on test material used for the study:

- Name of test material (as cited in study report): Piperazine citrate / tripiperazine dicitrate- Molecular formula: C12H30N6•Cl2H16O14 - Molecular weight: 642.76 g/mole - Substance type: Organic - Physical state: Solid

Analytical monitoring:

not specified

Details on sampling:

not specified

Vehicle:

yes

Details on test solutions:

The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 1 liters of potable water (passed through reverse osmosis system) with continuous stirring. This test solution was then added to the remaining three liters of water for achieving test concentrations of 100 mg/L.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM- Common name:Zebra Fish - Strain:Danio rerio- Source: Unique Aqua Fish Corner- Age at study initiation (mean and range, SD):not specified- Length at study initiation (length definition, mean, range and SD): average 0.180 g- Weight at study initiation (mean and range, SD): average 0.180 g- Method of breeding: not specified- Feeding during test: Once in a day up to sixth day of acclimatization- Food type:Standard brand feed,- Amount: not specified- Frequency: once daily

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

not specified

Test temperature:

23 °C

pH:

7.98

Dissolved oxygen:

7.4 mg/L

Salinity:

not specified

Conductivity:

not specified

Nominal and measured concentrations:

100 mg/L

Details on test conditions:

Photoperiod: 12 hours light and 12-hour darknessVolume of water: 2 litersNo. of animals: 8 fishesTest temperature: 23 °CAdaptation: Test conditions corresponded to housing conditionsTest datesTest started at : 05/03/2016Test Exposure date: 14/03/2016Date of experimental termination: 18/03/2016Withdrawal of food: 1 day before and during exposureAeration: Aeration in test vessels was provided 1 day before the start of experiment

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Observations:

Mortality and symptoms

NominalConcentration (mg/l)	Number of fish		Mortality (cumulated) after
			3 h		6 h		24h			48h		72h		96h
			V1	V2	V1	V2	V1	V2		V1	V2	V1	V2	V1	V2
0 (control)	4	4	-	-	-	-	-		-	-	-	-	-	-	-
100	4	4	-	-	-	-	-		-	-	-	-	-	-	-
100	4	4	-	-	-	-	-		-	-	-	-	-	-	-

Visible Symptoms:

NominalConcentration

(mg/l)

Number of fish

Symptoms after

3 h

6 h

24h

48h

72h

96h

0 (control)

NS4

100

NS4

100

NS4

Explanation of symptoms:

A:apathy

N:Narcotic-like state

C:Swimming near water surface

M: Slow movements compared to control

NS:No abnormal symptoms

S:Surfacing behaviour

L:Loss of equilibrium

SM:Shimming movement

H1:Hyperactivity

H2:Hypo activity

B:Bending behavior

F:Fin rot

IS:Invert Swimming

SB:Swimming behaviour

RF:Respiratory Function

P:Pigmentation

pH and oxygen content:

NominalConcentration (mg/l)	Number of fish		pH readings after
			24h			48h		72h		96h
			V1	V2		V1	V2	V1	V2	V1	V2
0 (control)	4	4	8.11		8.12	8.12	8.13	8.12	8.14	8.17	8.18
100	4	4	8.24		8.25	8.41	8.42	8.17	8.21	8.24	8.25
100	4	4	8.17		8.19	8.51	8.52	8.19	8.22	8.51	8.51

NominalConcentration (mg/l)	Number of fish		Oxygen content (mg/L) after
			24h			48h		72h		96h
			V1	V2		V1	V2	V1	V2	V1	V2
0 (control)	4	4	6.9		6.9	7.1	7.1	7.1	7.1	7.1	7.1
100	4	4	7.2		7.1	7.2	7.2	7.3	7.3	7.3	7.3
100	4	4	7.4		7.4	7.4	7.4	7.1	7.1	7.3	7.3

Test temperature

NominalConcentration (mg/l)	Number of fish		Temperature (°C) after
			24h			48h		72h		96h
			V1	V2		V1	V2	V1	V2	V1	V2
0 (control)	4	4	23		23	27	27	26	26	25	25
100	4	4	25		25	26	26	25	26	25	25
100	4	4	25		25	26	26	26	26	25	25

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentration, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No. -144-29-6 on Zebra fish (Danio rerio) was determined to be >100mg/L.

Executive summary:

Fish Acute Toxicity test according to OECD Guideline 203 was conducted for the test substance Tripiperazine dicitrate (CAS 144 -29 -6). The nominal concentration selected for the experiment was 100mg/L and Zebra Fish (Danio rerio) were exposed to thisconcentration for 96 hours.

The lethal concentrations LC50 was found to be >100mg/L.

Description of key information

Based on nominal concentration, experimental median lethal Concentrations [LC-50 (96 h)] for CAS No. -144-29-6 on Zebra fish (Danio rerio) was determined to be >100 mg/L. Based on this result it is concluded that the substance Piperazine citrate (CAS no. 144-29-6) does not qualify for the aquatic classification as per the CLP criteria.

For chemical safety assessment key value is considered to be 101 mg/L.

Key value for chemical safety assessment

Fresh water fish

Effect concentration:: 101 mg/L

Additional information

Short term toxicity to fish was assessed for the target compound Piperazine citrate (CAS no. 144-29-6) and its related read- across by reviewing a number of studies. The summary of the results are presented below:

In key study, Fish Acute Toxicity test according to OECD Guideline 203 was conducted for the test substanceTripiperazine dicitrate (CAS 144 -29 -6). The nominal concentration selected for the experiment was 100mg/L and Zebra Fish (Danio rerio) were exposed to thisconcentration for 96 hours.

The lethal concentrations LC50 was found to be >100mg/L.

Supporting study from reviewed journal DISEASES OF AQUATIC ORGANISMS, Vol. 10: 39-43, 1991 indicate that the in – vivo toxicity study, Gyrodactylus sp. infecting rainbow trout Oncorhynchus mykiss treated with Piperazine citrate in the concentration of 0 and 200 mg/l. No effect were observed on Percentage of reduction and number of dead (immobile) rainbow trout Oncorhynchus mykiss fish. Therefore, EC0 was considered to be 200 mg/l when Gyrodactylus sp. infecting rainbow trout Oncorhynchus mykiss treated with Piperazine citrate.

Whereas read across substance Piperazine (Cas no. 110-85-0) from Data bank of Environmental Properties of Chemicals (EnviChem), 2016 indicate that Fish toxicity study was conducted for 48 hrs for evaluating the lethal concentration (LC50)of test substance Piperazine on fish Oryzias latipes on the basis of mortality effect. During experiment the lethal concentration (LC50) for test substance Piperazine was found to be >1000 mg/l.

Same read across from European Union Risk Assessment Report – PIPERAZINE, 3rd Priority List Volume: 56;Final Report, 2005 the test chemical Piperazine (Cas no. 110-85-0) indicate semi-static test according to OECD Guidelines 203 (Balk and Meuwsen, 1989b). The test medium was renewed after 48 hours. The nominal test concentrations were 180, 320, 560,1,000 and 1,800 mg/L. Test temperature was 22.3-23°C, pH of the test medium was neutralised to 7.0-7.3. Observations of mortality and sublethal effects among the fish (10 per test concentration) were performed at daily intervals during the test. LC50 could be determined to be>1, 800 mg/L.

Based on above results for target chemical and its read across it is concluded that the substance Piperazine citrate (CAS no. 144-29-6) does not qualify for the aquatic classification as per the CLP criteria.

Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.