Trioctylphosphine

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From 1985-03-11 to 1985-03-29

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1985

Report date:

1985

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Trioctylphosphine

EC Number:

225-234-2

EC Name:

Trioctylphosphine

Cas Number:

4731-53-7

Molecular formula:

C24H51P

IUPAC Name:

trioctylphosphane

Test material form:

liquid

Test animals

Species:

rabbit

Strain:

New Zealand White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Age at study initiation: young adults (5 males and 1 female)
- Housing:Individually housed
- Cage: suspended, stainless steel
- Diet (e.g. ad libitum): Lab rabbit chow HF (Purine #5326)
- Water (e.g. ad libitum): Automatic watering system Municipal water supply (Elizabeth town water Co)
- Acclimation period: 15 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 15.6 °C - 21.1 °C (60 - 70 °F)
- Humidity (%): 30 - 70
- Photoperiod (hrs dark / hrs light): 12h / 12h

IN-LIFE DATES: From: To: 1985-03-26 to 1985-03-29

Test system

Type of coverage:

semiocclusive

Preparation of test site:

other: Clipped and abraded

Vehicle:

unchanged (no vehicle)

Controls:

no

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.5 mL

Duration of treatment / exposure:

Approximately 24h

Observation period:

72h

Number of animals:

6 (5 males and 1 female)

Details on study design:

TEST SITE
- Area of exposure: back (between scapular and lumbar region)
- % coverage: 2.54 cm² (1 x 1 inch)
- Type of wrap if used: Plastic sheeting was wrapped around the animal and secured with tape. Plastic sheeting was then wrapped around the animal and secured with tape to retard evaporation an keep the test substance in contact with the skin without undue pressure. Elizabethan collars were then placed on the animals to prevent disruption of the wrappings and ingestion of the test material.

REMOVAL OF TEST SUBSTANCE
- Washing (if done): The test sites were gently wiped free of excess test material with a paper towel and water.
- Time after start of exposure: 24h

OBSERVATION TIME POINTS
30 minutes after removal of wrappings and 72h after initiation of the treatment

SCORING SYSTEM:
- Method of calculation:

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

primary dermal irritation index (PDII)

Basis:

mean

Time point:

other: 24/72h

Score:

6.3

Max. score:

8

Reversibility:

not specified

Results 2

Irritation parameter:

erythema score

Basis:

mean

Time point:

24 h

Score:

3.7

Max. score:

4

Reversibility:

not specified

Results 3

Irritation parameter:

edema score

Basis:

mean

Time point:

24 h

Score:

2.8

Max. score:

4

Reversibility:

not specified

Results 4

Irritation parameter:

erythema score

Basis:

mean

Time point:

72 h

Score:

4

Max. score:

4

Reversibility:

not specified

Remarks on result:

positive indication of irritation

Results 5

Irritation parameter:

edema score

Basis:

mean

Time point:

72 h

Score:

2.1

Max. score:

4

Reversibility:

not specified

Any other information on results incl. tables

Individual dermal irritation scores – 24h and 72h

Time	Sites	Type	6443 M	6444 F	6469 M	6453 M	6445 M	6451 M	Mean score
24h	Right side (non-abraded)	Erythema	4S	4S	4S	3	3	4S	3.7
		Edema	3	3	3	2	3	3	2.8
	Left side (Abraded)	Erythema	4S	4S	4S	3	3	4S	3.7
		Edema	3	4	3	2	2	3	2.8
72h	Right side (non-abraded)	Erythema	4N	4N	4N	4N	4N	4N	4.0
		Edema	2	2	2	2	2	2	2.0
	Left side (Abraded)	Erythema	4	4N	4N	4N	4N	4N	4.0
		Edema	2	2	2	2	2	3	2.2
								Total	25.2
								PDII	6.3

 N : Necrosis

S : Superficial necrosis

Applicant's summary and conclusion

Interpretation of results:

Category 1 (corrosive) based on GHS criteria

Conclusions:

The substance was corrosive to the skin. At 24h, four of six animals had superficial necrosis with moderate or severe edema at both the intact and abraded sites and two had mederate to severe erythema and edema. By 72h, all six animal had necrosis et the intact and/or abraded sites.
The primary Iirritation Index is 6.3

Executive summary:

In an in vivo skin corrosion study on rabbits, 0.5 mL of trioctylphosphine was applied on intact or abraded skin of rabbit.

Observations at 24h and 72h after application showed edema, erythema and necrosis.

A PDII was calculated to be 6.3.

The substance was considered to be corrosive to the skin.