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EC number: 431-620-3
CAS number: 162537-11-3
Please refer to attached pdf document for detailed results.
Salmonella typhimurium strains TA1535, TA1537, TA98 and TA100 and
Eschericia coli strain WP2uvrA- were treated with the test material
using the Ames plate incorporation method at five dose levels, in
triplicate, both with and without the addition of a rat liver homogenate
metabolising system (10% liver S9 in standard co-factors). This method
conforms to the guidelines for bacterial mutagenicity testing published
by the major Japanese Regulatory Authorities including MITI, MHW, MOL
and MAFF. It also meets the requirements of the OECD, EC and USA EPA
(TSCA) guidelines. The dose range was determined in a preliminary
toxcity assay and was 50 to 5000 μg/plate in the first experiment. The
experiment was repeated on a separate day using the same dose range as
Experiment 1, fresh cultures of the bacterial strains and fresh test
Validity of test
The vehicle (dimethyl sulphoxide) control plates gave counts of
revertant colonies within the normal range.
All of the positive control chemicals used in the test induced marked
increases in the frequency of revertant colonies, both with and without
metabolic activation. Thus, the sensitivity of the assay and the
efficacy of the S9-mix were validated.
Results and conclusions
The test material caused no visible reduction in the growth of the
bacterial lawn at any dose level. The test material was, therefore,
tested up to the maximum recommended dose of 5000 μg/plate.
No significant increases in the frequency of revertant colonies were
recorded for any of the bacterial strains, with any dose of the test
material, either with or without metabolic activation. The test material
was considered to be non-mutagenic under the conditions of this test.
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