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EC number: 431-620-3
CAS number: 162537-11-3
A study was performed to assess the acute toxicity of the test material
to Daphnia magna. The method followed that described in the OECD
Guidelines for Testing of Chemicals (1984) No 202, "Daphnia sp, Acute
Immobilisation Test and Reproduction Test" referenced as Method C.2 of
Commission Directive 92/69/EEC (which constitutes Annex V of Council
Following a preliminary range-finding study, forty daphnids (4 replictes
of 10 animals) were exposed to an aqueous solution of the test material
at a concentration of 100 mg/l for 48 hours under static test
conditions. Immobilisation and any adverse reactions to exposure were
recorded after 24 and 48 hours.
The 48-hour EC50 for the test material to Daphnia magna based on nominal
test concentrations was greater than 100 mg/l and correspondingly the No
Observed Effect Concentration was 100 mg/l. It was considered
unnecessary and unrealistic to test at concentrations in excess of 100
mg/l. Analysis of the test solutions at 0 and 48 hours showed the
measured test concentrations to be near nominal and so the results are
based on nominal test concentrations only.
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