2-amino-4,6-dinitrophenol

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from Water Resources Bulletin

Data source

Reference

Reference Type:

publication

Title:

Acute Toxicity of Picric Acid and Picramic Acid to Rainbow Trout, Salmo Gairdneri, and American Oyster, Crassostrea Virginica’

Author:

William L. Goodfellow, Jr., Dennis T. Burton, William C. Graves, Lenwood W. Hall, Jr., and Keith R. Cooper

Year:

1983

Bibliographic source:

Water Resources Bulletin, VOL. 19, NO. 4-641-648, AUGUST 1983

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: as below

Principles of method if other than guideline:

Standard Methods (APHA, et al., 1981).

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2-amino-4,6-dinitrophenol

EC Number:

202-544-6

EC Name:

2-amino-4,6-dinitrophenol

Cas Number:

96-91-3

Molecular formula:

C6H5N3O5

IUPAC Name:

2-amino-4,6-dinitrophenol

Test material form:

other: Dark red needles

Details on test material:

- Name of test material (as cited in study report): Picramic acid
- Molecular formula (if other than submission substance): C6H5N3O5
- Molecular weight (if other than submission substance): 199.125g\mol
- Smiles notation (if other than submission substance): c1(cc(cc(c1O)N)[N+](=O)[O-])[N+](=O)[O-]
- InChl (if other than submission substance): 1S/C6H5N3O5/c7-4-1-3(8(11)12)2-5(6(4)10)9(13)14/h1-2,10H,7H2
- Substance type: Organic
- Physical state: Dark red needles
- Analytical purity: No data available.
- Lot/batch No.:38C-0117
- Storage condition of test material: The stock solutions were stored in the dark at 5°C.

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

yes

Test organisms

Test organisms (species):

other aquatic mollusc: Crassostrea virginica

Details on test organisms:

TEST ORGANISM
- Common name: American Or Virginia Oyster)
- Strain: virginica
- Source: Wilde Oyster Hatchery, Shady Side, Maryland
- Age at study initiation (mean and range, SD): Clutchless
- Weight at study initiation (mean and range, SD): No data available
- Length at study initiation (length definition, mean, range and SD): No data available
- Valve height at study initiation, for shell deposition study (mean and range, SD): 48.4 + 6.2 mm
- Peripheral shell growth removed prior to test initiation: No data available
- Method of breeding: No data available
- Feeding during test: Yes
- Food type: starch-yeast solution
- Amount: Fed 500 ml of 1.5 percent starch-yeast solution
- Frequency: Daily

ACCLIMATION
- Acclimation period: 2weeks
- Acclimation conditions (same as test or not): Yes
- Type and amount of food: Fed natural plankton in the estuarine water system supplemented with 1.5 percent corn starch-yeast solution when necessary.
- Feeding frequency: As per requirement.
- Health during acclimation (any mortality observed): Less than 3 percent mortality occurred in thelaboratory populations of oysters during acclimation.

Study design

Test type:

static

Water media type:

saltwater

Total exposure duration:

144 h

Test conditions

Test temperature:

10.5°C

pH:

7.6

Salinity:

7.0

Nominal and measured concentrations:

0, 5, 15, 25, 35, and 75 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass aquaria
- Type (delete if not applicable): closed
- Material, size, headspace, fill volume: 38 l
- Aeration: Air-lift system was provided.
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate): 24 hour
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates):6 concentration (one replicate)

TEST MEDIUM / WATER PARAMETERS
- Culture medium different from test medium: No
- Intervals of water quality measurement:3 days

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod: 12 hlight:12 h dark

Results and discussion

Effect concentrations

Duration:

144 h

Dose descriptor:

LC50

Effect conc.:

69.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Applicant's summary and conclusion

Conclusions:

LC50 was found to be 69.8 mg/l for Picramic acid in Crassostrea virginica for 144 hour

Executive summary:

In Long-term toxicity to aquatic invertebrates for Picramic acid was determined in Crassostrea virginica for 144 hour. The LC50 was found to be 69.8 mg/l for Picramic acid.