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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in mammalian cells
Remarks:
Type of genotoxicity: gene mutation
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
publication
Title:
Genotoxicity of vanadium compounds in yeast and cultured mammalian cells
Author:
Galli, A.et al.
Year:
1991
Bibliographic source:
Teratog Carcinog Mutagen. 11(4):175-83

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 476 (In Vitro Mammalian Cell Gene Mutation Test)
GLP compliance:
no
Type of assay:
mammalian cell gene mutation assay

Test material

Constituent 1
Reference substance name:
Vanadium oxide sulphate
EC Number:
248-652-7
EC Name:
Vanadium oxide sulphate
Cas Number:
27774-13-6
IUPAC Name:
oxovanadium(2+) sulfate
Details on test material:
- Name of test material (as cited in study report): vanadyl sulfate
- Supplier: Merck (Darmstadt, Germany)
- Analytical purity: no data
- Storage condition of test material: under nitrogen atmosphere

Method

Target gene:
HPRT locus
Species / strain
Species / strain / cell type:
Chinese hamster lung fibroblasts (V79)
Additional strain / cell type characteristics:
not specified
Metabolic activation:
with and without
Metabolic activation system:
mouse hepatic S-9 mix
Test concentrations with justification for top dose:
1, 2, 5,. 7.5 mM
Vehicle / solvent:
- Vehicle(s)/solvent(s) used: none
Controlsopen allclose all
Untreated negative controls:
yes
Negative solvent / vehicle controls:
no
True negative controls:
no
Positive controls:
yes
Positive control substance:
ethylmethanesulphonate
Remarks:
without metabolic activation

Migrated to IUCLID6: 30 mM
Untreated negative controls:
yes
Negative solvent / vehicle controls:
no
True negative controls:
no
Positive controls:
yes
Positive control substance:
N-dimethylnitrosamine
Remarks:
with metabolic activation

Migrated to IUCLID6: 10 mM
Details on test system and experimental conditions:
METHOD OF APPLICATION: in suspension


DURATION
- Expression time (cells in growth medium): 0, 144, 240 h

NUMBER OF REPLICATIONS: 4 independent experiments


NUMBER OF CELLS EVALUATED: 20^6


DETERMINATION OF CYTOTOXICITY
- Method: relative total growth
Statistics:
Student's t-test

Results and discussion

Test results
Species / strain:
Chinese hamster lung fibroblasts (V79)
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
cytotoxicity
Remarks:
cytotoxicity at highest concentration without metabolic activation
Vehicle controls validity:
not applicable
Untreated negative controls validity:
valid
Positive controls validity:
valid
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Any other information on results incl. tables

Induction of 6 -thioguanine resistance in V79 mammalian cells after vanadyl sulfate treatment with and without S-9 hepatic fraction at different expession times:

Concentration (mm) Survival 6TG resistants / 10^6 viable cells
without metabolic activation with metabolic activation without metabolic activation with metabolic activation
t0 t144 t244 t0 t144 t244
control 100 100 3.41 ± 1.21 3.86 ± 1.23 3.12 ± 1.10 5.27 ± 2.40 5.51 ± 2.39 5.34 ± 1.50
1 75 89 5.40 ± 1.46 3.92 ± 1.70 2.86 ± 0.91 7.36 ± 2.61 4.37 ± 1.82 3.20 ± 1.25
2 28 100 7.29 ± 3.67 2.68 ± 0.68 4.14 ± 2.01 6.31 ± 2.74 6.71 ± 2.09 1.35
5 20 100 3.28 ± 1.28 1.68 ± 0.20 1.98 ± 0.18 6.30 ± 1.09 9.10 ± 2.85 11.30 ± 4.96
7.5 tox 85 - - - 7.70 ± 2.30 10.70 ± 3.71 4.10 ± 1.94
EMS (30 mM) 52 - 4.22 ± 1.47 713 ± 32.6* - - - -
DMN (10 mM) - 60 -   - 4.29 ± 1.36 986 ± 152* -

*p<0.001

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative

DSD: not classified
CLP: not classified