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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Assessed after consultation with the relevant Authority. Data migrated from NONS files provided by Authority contain insufficient information.

Data source

Reference
Reference Type:
other: Body responsible for the test
Title:
Unnamed
Report date:
1991

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Qualifier:
according to guideline
Guideline:
EU Method B.1 (Acute Toxicity (Oral))
GLP compliance:
yes
Remarks:
PHARMACO-LSR LTD: EYE; SUFFOLK IP23 7PX
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Vanadyl pyrophosphate
EC Number:
406-260-5
EC Name:
Vanadyl pyrophosphate
Cas Number:
58834-75-6
Molecular formula:
O9P2V2
IUPAC Name:
vanadyl pyrophosphate
Details on test material:
- Analytical purity: no data

Test animals

Species:
rat
Strain:
Crj: CD(SD)
Sex:
male/female

Administration / exposure

Route of administration:
oral: unspecified
Vehicle:
other: 1.0% w/v methylcellulose in distilled water. The substance was administered as a suspension.
Doses:
1474, 2000, 2714, 3684, 5000 mg/kg bw
No. of animals per sex per dose:
5
Control animals:
not specified

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
2 171 mg/kg bw
95% CL:
1 789 - 2 552
Remarks on result:
other: Slope of the mortality curve: 68
Mortality:
Male: 1474 mg/kg bw; Number of animals: 5; Number of deaths: 1
Male: 2000 mg/kg bw; Number of animals: 5; Number of deaths: 2
Male: 2714 mg/kg bw; Number of animals: 5; Number of deaths: 4
Male: 3684 mg/kg bw; Number of animals: 5; Number of deaths: 5
Male: 5000 mg/kg bw; Number of animals: 5; Number of deaths: 5
Female: 1474 mg/kg bw; Number of animals: 5; Number of deaths: 1
Female: 2000 mg/kg bw; Number of animals: 5; Number of deaths: 0
Female: 2714 mg/kg bw; Number of animals: 5; Number of deaths: 5
Female: 3684 mg/kg bw; Number of animals: 5; Number of deaths: 5
Female: 5000 mg/kg bw; Number of animals: 5; Number of deaths: 4
Clinical signs:
Signs of toxicity related to dose levels:
Deaths occurred on days 2,3,4 and 6 (treatment was on day 1). Ante mortem signs comprised lethargy, decreased motor activity, prone posture, ataxia, muscle tremor, bradypnoea, hyperpnoea, piloerection, ungroomed appearance, pigmented staining of the snout, hunched posture, thin body conformation, diarrhoea and dark eyes.
Main signs of reaction in surviving animals were ungroomed appearance, diarrhoea and and thin body conformation.
These signs had resolved by day 5.
Gross pathology:
Effect on organs:
Necropsy of decedents showed altered stomach, small intestine and caecum contents and areas of body staining.
Surviving animals showed no significant macroscopic lesion.

Applicant's summary and conclusion

Interpretation of results:
not classified
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
DSD: not classified
CLP: not classified