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EC number: 224-832-0
CAS number: 4511-42-6
The influence of PURAC HS 88 on the respiration rate of activated sludge
was investigated after
a contact time of 3 hours.
The study procedure was based on OECD Guideline No. 209, adopted April
4, 1984, EEC Directive 67/548 amended November 18, 1987 (87/302), Part
C, Publication No. L133, adopted May 30, 1988 and ISO Standard 8192
The batch of PURAC HS 88 was a clear and colourless liquid that easily
dissolved in water. A stock solution of 500 mg/I was prepared by adding
126.0 mg test substance to 250 ml of Milli- RO water. A stirring period
of 30 minutes was used to accelerate dissolution and to ensure
homogeneity. Volumes of the stock solution corresponding to the test
concentration were then added to the test media. A concentration of 100
mg/I was tested in duplicate. Optimal contact between the test substance
and test medium was ensured applying continuous aeration and stirring.
No significant inhibition of respiration rate of the sludge was recorded
at 100 mg PURAC HS 88 per litre. The duplicate measurement confirmed the
result of the first measurement. Therefore no further testing was needed.
Since all criteria for acceptability of the test were met, this study
was considered to be valid.
In conclusion, under the conditions of this present test, PURAC HS 88
was not toxic to waste water (activated sludge) bacteria at a
concentration of 100 mg/I.
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