Zinc nitrate

Basic toxicokinetics

Administrative data

Endpoint:

basic toxicokinetics in vivo

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Study period:

Not reported

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study well documented, meets generally accepted scientific principles, acceptable for assessment

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1983

Materials and methods

Objective of study:

distribution

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Distribution of zinc to different organs measured for up to 14 d after single oral administration of radiolabelled zinc chloride to male Wistar rats.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Zinc chloride

EC Number:

231-592-0

EC Name:

Zinc chloride

Cas Number:

7646-85-7

Molecular formula:

Cl2Zn

IUPAC Name:

zinc(2+) ion dichloride

Details on test material:

- Name of test material (as cited in study report): Zinc chloride
- Locations of the label (if radiolabelling): Zn

Radiolabelling:

yes

Test animals

Species:

rat

Strain:

Wistar

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 240 g
- Diet: Granulated feed supplied by Bacutil and supplemented with white bred, milk and carrot.

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

not specified

Details on exposure:

No data

Duration and frequency of treatment / exposure:

Single administration

Doses / concentrations

Remarks:

Doses / Concentrations:
0.1 µCi (3.7 kBq) of Zn (65)

No. of animals per sex per dose / concentration:

30

Control animals:

other: not applicable

Positive control reference chemical:

Not applicable

Details on study design:

No data

Details on dosing and sampling:

PHARMACOKINETIC STUDY (Distribution)
- Tissues and body fluids sampled: Stomach, small intestine, large intestine without its contents, liver, kidneys, lungs, spleen, testicle, prostate, brain, blood, heart, thigh muscles and hairs.
- Time and frequency of sampling: After 6 and 24 h and after 2, 4, 7, 14 d from the administration of radioisotope.
- Other: To avoid any losses of Zn (65), radioactivity of 1 g of each sample (1 mL of blood , whole heart, spleen and prostate) was immediately measured.

Statistics:

Student's t test

Results and discussion

Preliminary studies:

Not performed

Main ADME results

Type:

distribution

Results:

Highest accumulation in small intestine, liver, kidneys and large intestine; smaller amount in lungs and spleen; smallest amounts at nearly same level in brain, prostate, heart, blood, skin, hairs and gonads.

Toxicokinetic / pharmacokinetic studies

Details on absorption:

Not measured

Details on distribution in tissues:

Highest accumulation in small intestine, liver, kidneys and large intestine; smaller amount in lungs and spleen; smallest amounts at nearly same level in brain, prostate, heart, blood, skin, hairs and gonads. (See Fig. 1-15 in the reference for details)

Details on excretion:

Not measured

Metabolite characterisation studies

Metabolites identified:

not measured

Details on metabolites:

Not applicable

Any other information on results incl. tables

None

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information): other:
In conclusion, zinc was mainly accumulated in small intestine, liver, kidneys and large intestine after single oral administration of zinc chloride to male Wistar rats.

Executive summary:

A study was conducted to determine the distribution of zinc to different organs after oral administration of zinc chloride in rats.

30 male Wistar rats were intubated with 0.1 µCi (3.7 kBq) ⁶⁵Zn as zinc chloride. Animals were sacrificed after 6 and 24 h and after 2, 4, 7, 14 d of administration and various organs viz. stomach, small intestine, large intestine without its contents, liver, kidneys, spleen, testicle, prostate, brain, blood, heart, thigh muscles and hairs were analysed for zinc concentration.

Zinc was accumulated in the highest amount within the small intestine, liver, kidneys and large intestine and in smaller amounts in lungs and spleen. The smallest amounts were found at nearly same level in brain, prostate, heart, blood, skin, hairs and gonads.

In conclusion, zinc was mainly accumulated in small intestine, liver, kidneys and large intestine after single oral administration of zinc chloride.